Home LiteratureArticle Details
PMID: 3009885 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Allosteric control of simian virus 40 T-antigen binding to viral origin DNA.

Journal of virology ·Vol. 58 ·No. 3 ·1986-06-00 ·Pages 765-72

Vogt B, Vakalopoulou E, Fanning E

Abstract

Simian virus 40 (SV40) large tumor antigen (T antigen) possesses several biochemical activities localized in different domains of the protein. These activities include sequence-specific binding to two major sites, I and II, in the SV40 control region, ATPase, and nucleotide-binding activity. In the present communication, we present evidence that specific binding of immunopurified T antigen to SV40 DNA is markedly inhibited by low concentrations of ATP, dATP, GTP, and dGTP. The inhibition is reversible after removal of the nucleotide, suggesting that simple nucleotide binding rather than a covalent modification of T antigen in the presence of ATP is responsible for the inhibition. The results suggest that T antigen may assume two conformations, one active and one inactive in binding to the SV40 origin of replication. In the presence of purine nucleoside triphosphates, the inactive conformation is favored.

MeSH Terms
Adenosine Diphosphate/pharmacology Adenosine Triphosphate/pharmacology Adenylyl Imidodiphosphate/pharmacology Allosteric Regulation Animals Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/metabolism Binding Sites Calcium/pharmacology DNA, Viral/metabolism Dose-Response Relationship, Drug Oncogene Proteins, Viral/metabolism Simian virus 40/metabolism
Chemicals
Antigens, Polyomavirus Transforming Antigens, Viral, Tumor DNA, Viral Oncogene Proteins, Viral Adenylyl Imidodiphosphate Adenosine Diphosphate Adenosine Triphosphate Calcium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Vogt B
Vakalopoulou E
Fanning E
References (47)
47 references, click to expand
  1. Minimal size plasmids containing an M13 origin for production of single-strand transducing particles.
    J Mol Appl Genet. 1984;2(6):507-17 PMID: 6099398
  2. T antigen repression of SV40 early transcription from two promoters.
    Cell. 1981 Dec;27(3 Pt 2):603-13 PMID: 6101224
  3. Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
    J Immunol. 1975 Dec;115(6):1617-24 PMID: 1102604
  4. Intramitochondrial and extramitochondrial concentrations of adenine nucleotides and inorganic phosphate in isolated hepatocytes from fasted rats.
    Eur J Biochem. 1978 Mar 15;84(2):413-20 PMID: 639797
  5. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose.
    Immunochemistry. 1978 Jul;15(7):429-36 PMID: 30693
  6. Evidence that glutamine, not sugar, is the major energy source for cultured HeLa cells.
    J Biol Chem. 1979 Apr 25;254(8):2669-76 PMID: 429309
  7. Regulation of DNA replication in S phase nuclei by ATP and ADP pools.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1643-7 PMID: 287004
  8. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  9. Trans-complementable copy-number mutants of plasmid ColE1.
    Nature. 1980 Jan 10;283(5743):216-8 PMID: 7350544
  10. Mutational analysis of the simian virus 40 replicon: pseudorevertants of mutants with a defective replication origin.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6128-31 PMID: 230499
  11. Cold-sensitive regulatory mutants of simian virus 40.
    J Mol Biol. 1980 Jun 15;140(1):129-42 PMID: 6251230
  12. Regulation of simian virus 40 early transcription in vitro by a purified tumor antigen.
    Proc Natl Acad Sci U S A. 1980 Oct;77(10):5706-10 PMID: 6255460
  13. SV40-transformed simian cells support the replication of early SV40 mutants.
    Cell. 1981 Jan;23(1):175-82 PMID: 6260373
  14. Detection and characterization of multiple forms of simian virus 40 large T antigen.
    J Virol. 1981 Jan;37(1):92-102 PMID: 6261004
  15. Mechanism of dnaB protein action. III. Allosteric role of ATP in the alteration of DNA structure by dnaB protein in priming replication.
    J Biol Chem. 1981 May 25;256(10):5260-6 PMID: 6262326
  16. Modification of SV40 T antigen by poly ADP-ribosylation.
    Cell. 1981 May;24(2):567-72 PMID: 7237559
  17. Heavy metal intensification of DAB-based HRP reaction product.
    J Histochem Cytochem. 1981 Jun;29(6):775 PMID: 7252134
  18. Purification and properties of a topoisomerase from Ustilago maydis.
    J Biol Chem. 1981 Oct 25;256(20):10354-61 PMID: 6270108
  19. Monoclonal antibodies specific for simian virus 40 tumor antigens.
    J Virol. 1981 Sep;39(3):861-9 PMID: 6169844
  20. Partial purification of SV40 A protein and a related cellular protein from permissive cells.
    Virology. 1981 Nov;115(1):67-74 PMID: 6270901
  21. Use of monoclonal antibodies as probes of simian virus 40 T antigen ATPase activity.
    J Biol Chem. 1981 Nov 25;256(22):11854-8 PMID: 6170639
  22. Equilibria and kinetics of lac repressor-operator interactions by polyacrylamide gel electrophoresis.
    Nucleic Acids Res. 1981 Dec 11;9(23):6505-25 PMID: 6275366
  23. Covalent affinity labeling by periodate-oxidized [alpha-32P]ATP of the large-T proteins of polyoma and SV40 viruses.
    J Biol Chem. 1982 Jun 10;257(11):6300-5 PMID: 6281268
  24. Discrete regions of simian virus 40 large T antigen are required for nonspecific and viral origin-specific DNA binding.
    J Virol. 1982 Jul;43(1):73-82 PMID: 6287017
  25. Regulatory mutants of simian virus 40. Effect of mutations at a T antigen binding site on DNA replication and expression of viral genes.
    J Mol Biol. 1982 Apr 15;156(3):531-48 PMID: 6288959
  26. Simian virus 40 large T antigen is phosphorylated at multiple sites clustered in two separate regions.
    J Virol. 1982 Oct;44(1):116-33 PMID: 6292479
  27. Improved localization of phosphorylation sites in simian virus 40 large T antigen.
    J Virol. 1983 Jan;45(1):315-31 PMID: 6296439
  28. Biochemical activities of T-antigen proteins encoded by simian virus 40 A gene deletion mutants.
    Mol Cell Biol. 1983 Feb;3(2):220-8 PMID: 6300658
  29. Specific DNA binding activity of T antigen subclasses varies among different SV40-transformed cell lines.
    Virology. 1983 Apr 15;126(1):19-31 PMID: 6302981
  30. Simian virus 40 tumor antigen: isolation of the origin-specific DNA-binding domain.
    J Virol. 1983 Jul;47(1):106-14 PMID: 6306267
  31. Essential contact residues within SV40 large T antigen binding sites I and II identified by alkylation-interference.
    Cell. 1984 Jan;36(1):155-62 PMID: 6319005
  32. Activation of the SV40 late promoter: direct effects of T antigen in the absence of viral DNA replication.
    Cell. 1984 Feb;36(2):381-9 PMID: 6319019
  33. Simian virus 40 mutant T antigens with relaxed specificity for the nucleotide sequence at the viral DNA origin of replication.
    J Virol. 1984 Feb;49(2):386-93 PMID: 6319739
  34. Structural and functional studies of the dnaB protein using limited proteolysis. Characterization of domains for DNA-dependent ATP hydrolysis and for protein association in the primosome.
    J Biol Chem. 1984 Jan 10;259(1):88-96 PMID: 6323419
  35. Subclasses of simian virus 40 large T antigen: differential binding of two subclasses of T antigen from productively infected cells to viral and cellular DNA.
    EMBO J. 1982;1(9):1023-8 PMID: 6329720
  36. Monoclonal antibodies against simian virus 40 nuclear large T tumour antigen: epitope mapping, papova virus cross-reaction and cell surface staining.
    EMBO J. 1984 Jul;3(7):1485-91 PMID: 6204863
  37. Specific in vitro adenylylation of the simian virus 40 large tumor antigen.
    Proc Natl Acad Sci U S A. 1984 Nov;81(21):6574-8 PMID: 6093107
  38. The nucleotide binding site detected by affinity labeling in the large T proteins of polyoma and SV40 viruses is distinct from their ATPase catalytic site.
    J Biol Chem. 1984 Dec 25;259(24):15196-203 PMID: 6096361
  39. Purification of simian virus 40 large T antigen by immunoaffinity chromatography.
    J Virol. 1985 Mar;53(3):1001-4 PMID: 2983081
  40. ATP-dependent specific binding of Tn3 transposase to Tn3 inverted repeats.
    Nature. 1985 Apr 11-17;314(6011):556-8 PMID: 2986006
  41. Seventeen base pairs of region I encode a novel tripartite binding signal for SV40 T antigen.
    Cell. 1985 Sep;42(2):539-48 PMID: 2992803
  42. trans Activation of the simian virus 40 late transcription unit by T-antigen.
    Mol Cell Biol. 1985 Jun;5(6):1391-9 PMID: 2993862
  43. Identification and biochemical analysis of DNA replication-defective large T antigens from SV40-transformed cells.
    Virology. 1985 Oct 30;146(2):188-202 PMID: 2996217
  44. Inhibition of HeLa cell DNA topoisomerase I by ATP and phosphate.
    Nucleic Acids Res. 1985 Oct 11;13(19):6999-7014 PMID: 2997732
  45. Antigenic binding sites of monoclonal antibodies specific for simian virus 40 large T antigen.
    J Virol. 1986 Mar;57(3):1168-72 PMID: 2419584
  46. Analysis of an activatable promoter: sequences in the simian virus 40 late promoter required for T-antigen-mediated trans activation.
    Mol Cell Biol. 1985 Aug;5(8):1859-69 PMID: 3018532
  47. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.58.3.765-772.1986
Published
1986-06-00
Pages
765-72
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252982
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.58.3.765-772.1986 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2