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PMID: 6319739 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Simian virus 40 mutant T antigens with relaxed specificity for the nucleotide sequence at the viral DNA origin of replication.

Journal of virology ·Vol. 49 ·No. 2 ·1984-02-00 ·Pages 386-93

Margolskee RF, Nathans D

Abstract

Base substitution of the ori region of simian virus 40 leads to plaque morphology mutants with markedly decreased DNA replication. Second-site mutations within the simian virus 40 T antigen gene suppress the plaque phenotype and replication defect of base-substituted ori mutants. Two second-site mutations have been mapped to a small segment of the T antigen gene, just beyond the distal splice junction. DNA sequence analysis revealed a single missense change in this segment of the T antigen gene of each of these second-site revertants, leading to a change in codon 157 in one case and codon 166 in the other. The mutant T antigens displayed relaxed specificity for the ori signal, i.e., they can function with several variously modified ori sequences, including those with small nucleotide deletions or insertions that are inactive for replication when coupled with wild-type T antigen. Thus a region of T antigen has been identified that appears to be intimately involved in vivo in binding to the ori sequence to initiate viral DNA replication.

MeSH Terms
Animals Antigens, Viral, Tumor/genetics Base Sequence Cell Line Chlorocebus aethiops Chromosome Deletion DNA Replication DNA, Recombinant/metabolism DNA, Viral/genetics Genes Genes, Viral Kidney Plasmids Simian virus 40/genetics,immunology
Chemicals
Antigens, Viral, Tumor DNA, Recombinant DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Margolskee R F
Nathans D
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42 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.49.2.386-393.1984
Published
1984-02-00
Pages
386-93
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255477
Subset
IM
Grants
NCI NIH HHS · 5 PO1 CA 16519 · United States
NIGMS NIH HHS · GM 07309 · United States
External Links
PubMed source DOI: 10.1128/JVI.49.2.386-393.1984 PMC Full Text View journal details
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