Home LiteratureArticle Details
PMID: 6285001 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Mutant of simian virus 40 large T-antigen that is defective for viral DNA synthesis, but competent for transformation of cultured rat cells.

Journal of virology ·Vol. 42 ·No. 3 ·1982-06-00 ·Pages 854-64

Stringer JR

Abstract

A mutant was isolated which demonstrates that the transforming activity of simian virus 40 large T-antigen is separable from its function in viral DNA replication. The mutant, SVR9D, is nonconditionally defective for viral DNA synthesis, but competent at wild-type level for morphological transformation of cultured rat cells. The lytic growth defect in SVR9D is complemented by the simian virus 40 A gene product present in the transformed CV1 cell line, COS1. The lesion in SVR9D DNA was mapped genetically by marker rescue of plaque formation and localized to a 214-base-pair segment of the viral genome bounded by nucleotide numbers 4100 and 4314. DNA sequence analysis showed the mutation to be an adenine-to-guanine transition at nucleotide number 4178. This change predicts a lysine-to-glutamic acid amino acid change at residue number 214 of the mutant large T-antigen polypeptide.

MeSH Terms
Animals Antigens, Viral/genetics Antigens, Viral, Tumor Base Sequence Cell Line Cell Transformation, Viral DNA Replication DNA, Viral/biosynthesis,genetics Genes, Viral Mutation Simian virus 40/genetics,immunology
Chemicals
Antigens, Viral Antigens, Viral, Tumor DNA, Viral
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Stringer J R
References (31)
31 references, click to expand
  1. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  2. Simian virus 40 deoxyribonucleic acid synthesis: the viral replicon.
    J Virol. 1972 Oct;10(4):591-8 PMID: 4343541
  3. Plasminogen activator production accompanies loss of anchorage regulation in transformation of primary rat embryo cells by simian virus 40.
    Proc Natl Acad Sci U S A. 1974 Dec;71(12):4792-6 PMID: 4373730
  4. T antigen binds to simian virus 40 DNA at the origin of replication.
    Proc Natl Acad Sci U S A. 1975 Apr;72(4):1605-9 PMID: 165517
  5. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  6. "Early" simian-virus-40-specific RNA contains information for tumor antigen formation and chromatin replication.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):366-70 PMID: 174105
  7. Identification of regions of the SV40 genome which contain preferred SV40 T antigen-binding sites.
    Cell. 1976 Aug;8(4):535-45 PMID: 182383
  8. The arrangement of simian virus 40 sequences in the DNA of transformed cells.
    Cell. 1976 Oct;9(2):269-87 PMID: 184963
  9. Properties of permissive monkey cells transformed by UV-irradiated simian virus 40.
    J Virol. 1977 May;22(2):256-66 PMID: 194053
  10. The binding site on SV40 DNA for a T antigen-related protein.
    Cell. 1978 Jan;13(1):165-79 PMID: 202398
  11. Transformation of BALB/c-3T3 cells by tsA mutants of simian virus 40: temperature sensitivity of the transformed phenotype and retransofrmation by wild-type virus.
    J Virol. 1978 Mar;25(3):860-70 PMID: 205675
  12. Biological activity of purified simian virus 40 T antigen proteins.
    Proc Natl Acad Sci U S A. 1978 Mar;75(3):1279-83 PMID: 206892
  13. Local mutagenesis: a method for generating viral mutants with base substitutions in preselected regions of the viral genome.
    Proc Natl Acad Sci U S A. 1978 May;75(5):2170-4 PMID: 209457
  14. Studies on simian virus 40 excision from cellular chromosomes.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:709-19 PMID: 226315
  15. Mutational analysis of the simian virus 40 replicon: pseudorevertants of mutants with a defective replication origin.
    Proc Natl Acad Sci U S A. 1979 Dec;76(12):6128-31 PMID: 230499
  16. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  17. Mapping simian virus 40 mutants by marker rescue.
    Methods Enzymol. 1980;65(1):811-6 PMID: 6246376
  18. Assay of transforming activity of tumor virus DNA.
    Methods Enzymol. 1980;65(1):826-39 PMID: 6246378
  19. Ubiquitous, interspersed repeated sequences in mammalian genomes.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1398-402 PMID: 6246492
  20. Integration and excision of SV40 DNA from the chromosome of a transformed cell.
    Cell. 1980 May;20(1):143-52 PMID: 6248231
  21. Origin-defective mutants of SV40.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:293-300 PMID: 6253143
  22. Requirement for the large T and small T proteins of SV40 in the maintenance of the transformed state.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:325-31 PMID: 6253147
  23. Amplification and rearrangement of integrated SV40 DNA sequences accompany the selection of anchorage-independent transformed mouse cells.
    Cell. 1980 Nov;22(2 Pt 2):535-43 PMID: 6256084
  24. SV40-transformed simian cells support the replication of early SV40 mutants.
    Cell. 1981 Jan;23(1):175-82 PMID: 6260373
  25. Separation of lytic and transforming functions of the simian virus 40 A region: two mutants which are temperature sensitive for lytic functions have opposite effects on transformation.
    J Virol. 1981 May;38(2):518-28 PMID: 6264125
  26. Integrated simian virus 40 DNA: nucleotide sequences at cell-virus recombinant junctions.
    J Virol. 1981 May;38(2):671-9 PMID: 6264135
  27. DNA sequences similar to those around the simian virus 40 origin of replication are present in the monkey genome.
    Proc Natl Acad Sci U S A. 1981 Jan;78(1):95-9 PMID: 6264457
  28. Characterization of a temperature-sensitive, DNA-positive, nontransforming mutant of simian virus 40.
    Virology. 1981 Jul 30;112(2):605-24 PMID: 6266143
  29. Binding of a simian virus 40 T antigen-related protein to DNA.
    J Mol Biol. 1981 Jan 25;145(3):471-88 PMID: 6267291
  30. Normal rat cell lines deficient in nuclear thymidine kinase.
    Virology. 1981 Aug;113(1):408-11 PMID: 7269249
  31. The transformation of cell growth and transmogrification of DNA synthesis by simian virus 40.
    Adv Cancer Res. 1981;34:1-68 PMID: 6269370
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.42.3.854-864.1982
Published
1982-06-00
Pages
854-64
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC256919
Subset
IM
Grants
NCI NIH HHS · CA-06460 · United States
NCI NIH HHS · CA-13106 · United States
External Links
PubMed source DOI: 10.1128/JVI.42.3.854-864.1982 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2