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PMID: 6264135 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Integrated simian virus 40 DNA: nucleotide sequences at cell-virus recombinant junctions.

Journal of virology ·Vol. 38 ·No. 2 ·1981-05-00 ·Pages 671-9

Stringer JR

Abstract

DNA fragments containing the integrated viral DNA present in the simian virus 40 (SV40)-transformed rat cell lines SVRE9 and SVRE17 were cloned in procaryotic vectors, and the DNA sequences linking SV40 and cell DNA were determined. Comparison of the DNA sequences at the SV40-cell junctions in SVRE9 and SVRE17 cells with those of a previously characterized viral insertion from SV14B cells shows that no specific viral or cellular sequences occur at SV40-cell junctions and that the cellular DNA sequences adjacent to integrated SV40 DNA do not display the direct repeat structure characteristic of transposons and retrovirus proviruses.

MeSH Terms
Animals Base Sequence Cell Line Cell Transformation, Viral Cloning, Molecular DNA DNA, Viral Rats Recombination, Genetic Repetitive Sequences, Nucleic Acid Simian virus 40/genetics
Chemicals
DNA, Viral DNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Stringer J R
References (26)
26 references, click to expand
  1. Insertion of the eukaryotic transposable element Ty1 creates a 5-base pair duplication.
    Nature. 1980 Jul 24;286(5771):352-6 PMID: 6250062
  2. Sequence of retrovirus provirus resembles that of bacterial transposable elements.
    Nature. 1980 Jun 19;285(5766):550-4 PMID: 6250038
  3. Integration of the simian virus 40 genome into cellular DNA in temperature-sensitive (N) and temperature-insensitive (A) transformants of 3T3 rat and Chinese hamster lung cells.
    J Virol. 1980 Jul;35(1):184-93 PMID: 6251267
  4. Two types of somatic recombination are necessary for the generation of complete immunoglobulin heavy-chain genes.
    Nature. 1980 Aug 14;286(5774):676-83 PMID: 6774258
  5. Transposable elements.
    Cell. 1980 Jul;20(3):579-95 PMID: 6251969
  6. Analysis of the sites of integration of viral DNA sequences in rat cells transformed by adenovirus 2 or SV40.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:569-84 PMID: 6253158
  7. Nucleotide sequences of integrated Moloney sarcoma provirus long terminal repeats and their host and viral junctions.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):3937-41 PMID: 6254003
  8. Insertion of the Drosophila transposable element copia generates a 5 base pair duplication.
    Cell. 1980 Sep;21(2):575-9 PMID: 6250725
  9. Plasminogen activator production accompanies loss of anchorage regulation in transformation of primary rat embryo cells by simian virus 40.
    Proc Natl Acad Sci U S A. 1974 Dec;71(12):4792-6 PMID: 4373730
  10. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  11. Integrated simian virus 40 sequences in transformed cell DNA: analysis using restriction endonucleases.
    Proc Natl Acad Sci U S A. 1976 Apr;73(4):1102-6 PMID: 177975
  12. The arrangement of simian virus 40 sequences in the DNA of transformed cells.
    Cell. 1976 Oct;9(2):269-87 PMID: 184963
  13. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  14. EK2 derivatives of bacteriophage lambda useful in the cloning of DNA from higher organisms: the lambdagtWES system.
    Science. 1977 Apr 8;196(4286):175-7 PMID: 322278
  15. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  16. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  17. Characterization and sequence analysis of a recombination site in the hybrid virus Ad2+ND.
    J Mol Biol. 1978 Mar 25;120(1):13-31 PMID: 205651
  18. Complete nucleotide sequence of SV40 DNA.
    Nature. 1978 May 11;273(5658):113-20 PMID: 205802
  19. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  20. Cloning human fetal gamma globin and mouse alpha-type globin DNA: preparation and screening of shotgun collections.
    Science. 1978 Dec 22;202(4374):1279-84 PMID: 725603
  21. Evolutionary variants of simian virus 40: Cellular DNA sequences and sequences at recombinant joints of substituted variants.
    J Mol Biol. 1978 Dec 5;126(2):275-88 PMID: 216811
  22. Transformation of mammalian cells with genes from procaryotes and eucaryotes.
    Cell. 1979 Apr;16(4):777-85 PMID: 222468
  23. Studies on simian virus 40 excision from cellular chromosomes.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:709-19 PMID: 226315
  24. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  25. Integration and excision of SV40 DNA from the chromosome of a transformed cell.
    Cell. 1980 May;20(1):143-52 PMID: 6248231
  26. The yeast transposon Ty1 generates duplications of target DNA on insertion.
    Nature. 1980 Jul 24;286(5771):414-8 PMID: 6250065
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.38.2.671-679.1981
Published
1981-05-00
Pages
671-9
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC171197
Subset
IM
Grants
NCI NIH HHS · CA06460-01 · United States
External Links
PubMed source DOI: 10.1128/JVI.38.2.671-679.1981 PMC Full Text View journal details
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