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PMID: 2983081 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Purification of simian virus 40 large T antigen by immunoaffinity chromatography.

Journal of virology ·Vol. 53 ·No. 3 ·1985-03-00 ·Pages 1001-4

Dixon RA, Nathans D

Abstract

Simian virus 40 large T antigen from lytically infected cells has been purified to near homogeneity by immunochromatography of the cell extract on a protein A-Sepharose-monoclonal antibody column. The resulting T antigen retains biochemical activity; i.e., it hydrolyzes ATP and binds to simian virus 40 DNA at the origin of replication.

MeSH Terms
Animals Antibodies, Monoclonal/immunology Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/immunology,isolation & purification Chromatography, Affinity Haplorhini Simian virus 40/immunology Viral Proteins/immunology,isolation & purification
Chemicals
Antibodies, Monoclonal Antigens, Polyomavirus Transforming Antigens, Viral, Tumor Viral Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dixon R A
Nathans D
References (10)
10 references, click to expand
  1. Initiation of simian virus 40 DNA replication in vitro.
    J Virol. 1983 Nov;48(2):481-91 PMID: 6312104
  2. Simian virus 40 DNA replication in vitro.
    Proc Natl Acad Sci U S A. 1984 Nov;81(22):6973-7 PMID: 6095264
  3. The isolation of simian virus 40 variants with specifically altered genomes.
    Proc Natl Acad Sci U S A. 1974 Mar;71(3):942-6 PMID: 4362640
  4. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  5. A one-step purification of membrane proteins using a high efficiency immunomatrix.
    J Biol Chem. 1982 Sep 25;257(18):10766-9 PMID: 6955305
  6. Protein-DNA interactions at the origin of simian virus 40 DNA replication.
    Cold Spring Harb Symp Quant Biol. 1979;43 Pt 2:655-61 PMID: 226310
  7. Catalytic properties of the SV40 large T antigen.
    Cold Spring Harb Symp Quant Biol. 1980;44 Pt 1,:103-11 PMID: 6253123
  8. Binding of a simian virus 40 T antigen-related protein to DNA.
    J Mol Biol. 1981 Jan 25;145(3):471-88 PMID: 6267291
  9. Monoclonal antibodies specific for simian virus 40 tumor antigens.
    J Virol. 1981 Sep;39(3):861-9 PMID: 6169844
  10. The use of thin acrylamide gels for DNA sequencing.
    FEBS Lett. 1978 Mar 1;87(1):107-10 PMID: 631324
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.53.3.1001-1004.1985
Published
1985-03-00
Pages
1001-4
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC254743
Subset
IM
Grants
NCI NIH HHS · 5 PO1 CA16519 · United States
External Links
PubMed source DOI: 10.1128/JVI.53.3.1001-1004.1985 PMC Full Text View journal details
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