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PMID: 2419584 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Antigenic binding sites of monoclonal antibodies specific for simian virus 40 large T antigen.

Journal of virology ·Vol. 57 ·No. 3 ·1986-03-00 ·Pages 1168-72

Gurney EG, Tamowski S, Deppert W

Abstract

We isolated 16 new monoclonal antibodies that recognize large T antigen of simian virus 40 and mapped the epitopes to three distinct regions of the large T antigen. Also, 3 of the 16 recognized the large T antigen of the human papovavirus BKV.

MeSH Terms

Animals Antibodies, Monoclonal/immunology Antigens, Polyomavirus Transforming Antigens, Viral, Tumor/analysis,immunology Cross Reactions Epitopes/analysis HeLa Cells Humans Immunoglobulin G/analysis Mice Mice, Inbred BALB C Oncogene Proteins, Viral/analysis,immunology Papillomaviridae/immunology Polyomaviridae Protein Denaturation

Chemicals

Antibodies, Monoclonal Antigens, Polyomavirus Transforming Antigens, Viral, Tumor Epitopes Immunoglobulin G Oncogene Proteins, Viral

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Gurney E G

Tamowski S

Deppert W

References (12)

12 references, click to expand

Continuous cultures of fused cells secreting antibody of predefined specificity.
Nature. 1975 Aug 7;256(5517):495-7 PMID: 1172191
Rapid isolation of antigens from cells with a staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A.
J Immunol. 1975 Dec;115(6):1617-24 PMID: 1102604
Evidence for simian virus 40 (SV40) coding of SV40 T-antigen and the SV40-specific proteins in HeLa cells infected with nondefective adenovirus type 2-SV40 hybrid viruses.
J Virol. 1977 Oct;24(1):151-69 PMID: 198574
An immunochemical investigation of SV40 T antigens. 1. Production properties and specificity of rabbit antibody to purified simian virus 40 large-T antigen.
Virology. 1978 Jun 1;87(1):182-93 PMID: 78572
Precise determination of the entire antigenic structure of lysozyme: molecular features of protein antigenic structures and potential of "surface-simulation" synthesis--a powerful new concept for protein binding sites.
Immunochemistry. 1978 Dec;15(12):909-36 PMID: 86497
Monoclonal antibodies against simian virus 40 nuclear large T tumour antigen: epitope mapping, papova virus cross-reaction and cell surface staining.
EMBO J. 1984 Jul;3(7):1485-91 PMID: 6204863
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
The genome of human papovavirus BKV.
Cell. 1979 Dec;18(4):963-77 PMID: 229976
SV40 large T shares an antigenic determinant with a cellular protein of molecular weight 68,000.
Nature. 1980 Nov 13;288(5787):167-70 PMID: 6159551
Analysis of the 84K, 55K, and 48K proteins immunoprecipitable by SV40 T antibody from SV40-infected and -transformed cells by tryptic peptide mapping on cation-exchange columns.
Virology. 1981 May;111(1):283-8 PMID: 6263006
Monoclonal antibodies specific for simian virus 40 tumor antigens.
J Virol. 1981 Sep;39(3):861-9 PMID: 6169844
Cell surface location of simian virus 40-specific proteins on HeLa cells infected with adenovirus type 2-simian virus 40 hybrid viruses Ad2+ND1 and Ad2+ND2.
J Virol. 1979 Aug;31(2):522-36 PMID: 90174

Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.57.3.1168-1172.1986

Published

1986-03-00

Pages

1168-72

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC252854

Subset

Grants

NCI NIH HHS · CA-21797 · United States

External Links

PubMed source DOI: 10.1128/JVI.57.3.1168-1172.1986 PMC Full Text View journal details

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