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PMID: 3023962 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Simian virus 40 DNA replication: functional organization of regulatory elements.

Molecular and cellular biology ·Vol. 6 ·No. 9 ·1986-09-00 ·Pages 3086-93

Lee-Chen GJ, Woodworth-Gutai M

Abstract

The efficiency of simian virus 40 (SV40) DNA replication is dependent on the structural organization of the regulatory region. The enhancing effect of the G + C-rich 21-base-pair (bp) repeats on SV40 DNA replication is position and dose dependent and to some extent orientation dependent. The inverted orientation is about 50% as effective as the normal orientation of the 21-bp repeat region. Movement of the 21-bp repeat region 180 or 370 bp upstream of the ori sequence abolishes its enhancing effect, whereas no replication is detected if the 21-bp repeat region is placed downstream of the ori sequence. The dose-dependent enhancement of the 21-bp repeat of SV40 DNA replication as first described in single transfection by Bergsma et al. (D. J. Bergsma, D. M. Olive, S. W. Hartzell, and K. N. Subramanian, Proc. Natl. Acad. Sci. USA 79:381-385, 1982) is dramatically amplified in mixed transfection. In the presence of the 21-bp repeat region, the 72-bp repeat region can enhance SV40 DNA replication. In the presence of the 21-bp repeats and a competitive environment, the 72-bp repeat region exhibits a cis-acting inhibitory effect on SV40 DNA replication.

MeSH Terms
Animals Base Composition Cell Line Chlorocebus aethiops DNA Replication DNA Restriction Enzymes Genes, Regulator Genes, Viral Genetic Vectors Kidney Plasmids Repetitive Sequences, Nucleic Acid Simian virus 40/genetics
Chemicals
DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lee-Chen G J
Woodworth-Gutai M
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55 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.6.9.3086-3093.1986
Published
1986-09-00
Pages
3086-93
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC367043
Subset
IM
Grants
NCI NIH HHS · CA-28250 · United States
External Links
PubMed source DOI: 10.1128/mcb.6.9.3086-3093.1986 PMC Full Text View journal details
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