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PMID: 6298741 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

High efficiency polyoma DNA transfection of chloroquine treated cells.

Nucleic acids research ·Vol. 11 ·No. 5 ·1983-03-11 ·Pages 1295-308

Luthman H, Magnusson G

Abstract

Chloroquine treatment of rodent cells during the first hours of polyoma DNA transfection increase the fraction of cells expressing viral functions. The effect has been observed after DNA absorption using both the DEAE-dextran and calcium phosphate coprecipitation methods. Exposure to chloroquine increased the proportion of transfected mouse cells to approximately 40%. From a culture of one million such cells, microgram quantities of newly synthesized viral DNA could be isolated. Similarly, the transformation frequency of rat cells following polyoma DNA transfection was approximately 6-fold increased by chloroquine treatment. The effect of the compound was even more pronounced in transfections with linear forms of polyoma DNA, suggesting that chloroquine inhibits degradation of DNA absorbed by the cells.

MeSH Terms
Animals Antigens, Polyomavirus Transforming Cell Line Cells, Cultured Chloroquine/pharmacology DNA Replication/drug effects DNA, Viral/genetics,isolation & purification Kinetics Mice Polyomavirus/drug effects,genetics Rats Transfection/drug effects Viral Proteins/genetics Virus Replication/drug effects
Chemicals
Antigens, Polyomavirus Transforming DNA, Viral Viral Proteins Chloroquine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Luthman H
Magnusson G
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26 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.5.1295
Published
1983-03-11
Pages
1295-308
Language
English
Region
England
NLM ID
0411011
PMCID
PMC325797
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/11.5.1295 PMC Full Text View journal details
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