Home LiteratureArticle Details
PMID: 8112311 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Strand and face: the topography of interactions between the SV40 origin of replication and T-antigen during the initiation of replication.

The EMBO journal ·Vol. 13 ·No. 4 ·1994-02-15 ·Pages 982-92

SenGupta DJ, Borowiec JA

Abstract

The mechanism by which a replicator (origin of replication) becomes denatured during the initiation of replication is not understood for any prokaryotic or eukaryotic system. To address this question, we chemically probed the molecular contacts on the SV40 origin of replication (ori) that are used by the SV40 large T-antigen and a single-stranded DNA-binding protein (SSB) during ori denaturation. Prior to the actual denaturation step, the T-antigen double hexamer bound ori utilizing sugar-phosphate contacts that were located on opposite strands in each flanking domain of ori. Each set of flanking phosphate contacts were also located on approximately opposite faces of the ori duplex. While the phosphate contacts had a 2-fold symmetry with respect to the ori center, T-antigen contacts with nucleotide bases were polar with critical interactions detected in only one of the two flanking domains. During origin denaturation catalyzed by T-antigen and a SSB, numerous new contacts to flanking phosphates were observed on the strand not initially bound by T-antigen, suggesting movement of each T-antigen hexamer outward from ori. These data suggest that T-antigen initially binds ori in a manner that facilitates transfer of each T-antigen hexamer to opposite strands during the initiation of SV40 replication.

MeSH Terms
Animals Antigens, Polyomavirus Transforming/metabolism Baculoviridae/genetics Base Sequence Cell Line DNA Replication DNA, Single-Stranded/metabolism DNA, Viral/biosynthesis,chemistry,metabolism DNA-Binding Proteins/metabolism Molecular Sequence Data Moths Nucleic Acid Conformation Nucleic Acid Denaturation Phosphates/metabolism Protein Binding Replicon Simian virus 40/genetics
Chemicals
Antigens, Polyomavirus Transforming DNA, Single-Stranded DNA, Viral DNA-Binding Proteins Phosphates
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
SenGupta D J
Department of Biochemistry, New York University Medical Center, NY 10016.
Borowiec J A
References (48)
48 references, click to expand
  1. Contacts between Escherichia coli RNA polymerase and an early promoter of phage T7.
    Proc Natl Acad Sci U S A. 1980 Jan;77(1):122-6 PMID: 6987648
  2. Recognition of model DNA replication forks by the SV40 large tumor antigen.
    Chromosoma. 1992;102(1 Suppl):S46-51 PMID: 1337879
  3. Torsional rigidity of DNA and length dependence of the free energy of DNA supercoiling.
    J Mol Biol. 1984 Feb 15;173(1):75-91 PMID: 6321743
  4. Monomers through trimers of large tumor antigen bind in region I and monomers through tetramers bind in region II of simian virus 40 origin of replication DNA as stable structures in solution.
    Proc Natl Acad Sci U S A. 1985 Jun;82(11):3626-30 PMID: 2987937
  5. Chemical probes of DNA conformation: detection of Z-DNA at nucleotide resolution.
    Cell. 1985 Oct;42(3):713-24 PMID: 4053183
  6. Functional interactions of the simian virus 40 core origin of replication with flanking regulatory sequences.
    J Virol. 1986 Jan;57(1):138-44 PMID: 3001340
  7. Extensive unwinding of the plasmid template during staged enzymatic initiation of DNA replication from the origin of the Escherichia coli chromosome.
    Cell. 1986 Apr 11;45(1):53-64 PMID: 3006926
  8. DNA helicase activity of SV40 large tumor antigen.
    EMBO J. 1986 Aug;5(8):1939-44 PMID: 3019672
  9. Specialized nucleoprotein structures at the origin of replication of bacteriophage lambda: localized unwinding of duplex DNA by a six-protein reaction.
    Proc Natl Acad Sci U S A. 1986 Oct;83(20):7638-42 PMID: 3020552
  10. Domain structure of the simian virus 40 core origin of replication.
    Mol Cell Biol. 1986 May;6(5):1663-70 PMID: 3023900
  11. Simian virus 40 (SV40) DNA replication: SV40 large T antigen unwinds DNA containing the SV40 origin of replication.
    Proc Natl Acad Sci U S A. 1987 Jan;84(1):16-20 PMID: 3025851
  12. The T-antigen-binding domain of the simian virus 40 core origin of replication.
    J Virol. 1987 Jul;61(7):2143-9 PMID: 3035215
  13. Initiation of simian virus 40 DNA replication in vitro: large-tumor-antigen- and origin-dependent unwinding of the template.
    Proc Natl Acad Sci U S A. 1987 Jun;84(11):3643-7 PMID: 3035543
  14. Missing contact probing of DNA-protein interactions.
    Proc Natl Acad Sci U S A. 1987 Oct;84(19):6673-6 PMID: 2958845
  15. ATP enhances the binding of simian virus 40 large T antigen to the origin of replication.
    J Virol. 1987 Dec;61(12):3649-54 PMID: 2824805
  16. Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.
    Proc Natl Acad Sci U S A. 1987 Dec;84(23):8267-71 PMID: 2825183
  17. The unwinding of duplex regions in DNA by the simian virus 40 large tumor antigen-associated DNA helicase activity.
    J Biol Chem. 1988 Jan 5;263(1):383-92 PMID: 2826443
  18. Simian virus 40 large T antigen DNA helicase. Characterization of the ATPase-dependent DNA unwinding activity and its substrate requirements.
    J Biol Chem. 1988 Jan 5;263(1):436-42 PMID: 2826446
  19. ATP-dependent formation of a specialized nucleoprotein structure by simian virus 40 (SV40) large tumor antigen at the SV40 replication origin.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):8981-5 PMID: 2827164
  20. ATP stimulates the binding of simian virus 40 (SV40) large tumor antigen to the SV40 origin of replication.
    Proc Natl Acad Sci U S A. 1988 Jan;85(1):64-8 PMID: 2829177
  21. Initiation protein induced helix destabilization at the lambda origin: a prepriming step in DNA replication.
    Cell. 1988 Feb 12;52(3):385-95 PMID: 2830983
  22. Duplex opening by dnaA protein at novel sequences in initiation of replication at the origin of the E. coli chromosome.
    Cell. 1988 Mar 11;52(5):743-55 PMID: 2830993
  23. Localized melting and structural changes in the SV40 origin of replication induced by T-antigen.
    EMBO J. 1988 Oct;7(10):3149-58 PMID: 2846276
  24. ATP-dependent assembly of double hexamers of SV40 T antigen at the viral origin of DNA replication.
    Nature. 1989 Apr 20;338(6217):658-62 PMID: 2539565
  25. Animal virus DNA replication.
    Annu Rev Biochem. 1989;58:671-717 PMID: 2549858
  26. Synthesis of DNA containing the simian virus 40 origin of replication by the combined action of DNA polymerases alpha and delta.
    Proc Natl Acad Sci U S A. 1989 Oct;86(19):7361-5 PMID: 2571990
  27. Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication.
    Nature. 1989 Nov 2;342(6245):92-5 PMID: 2554144
  28. Multiple replication factors augment DNA synthesis by the two eukaryotic DNA polymerases, alpha and delta.
    EMBO J. 1989 Dec 1;8(12):3883-9 PMID: 2573521
  29. Initiation of eukaryotic DNA replication in vitro.
    Annu Rev Cell Biol. 1989;5:197-245 PMID: 2557059
  30. Requirement for two DNA polymerases in the replication of simian virus 40 DNA in vitro.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):9742-6 PMID: 2574863
  31. Multiple functions of human single-stranded-DNA binding protein in simian virus 40 DNA replication: single-strand stabilization and stimulation of DNA polymerases alpha and delta.
    Proc Natl Acad Sci U S A. 1989 Dec;86(24):9757-61 PMID: 2557626
  32. Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen.
    J Virol. 1990 Feb;64(2):509-18 PMID: 2153220
  33. Binding and unwinding--how T antigen engages the SV40 origin of DNA replication.
    Cell. 1990 Jan 26;60(2):181-4 PMID: 2153460
  34. The in vitro replication of DNA containing the SV40 origin.
    J Biol Chem. 1990 Oct 25;265(30):18043-6 PMID: 1976634
  35. Differential induction of structural changes in the simian virus 40 origin of replication by T antigen.
    J Virol. 1991 Mar;65(3):1228-35 PMID: 1847451
  36. Simian virus 40 large T antigen untwists DNA at the origin of DNA replication.
    J Biol Chem. 1991 Mar 15;266(8):5062-71 PMID: 1848235
  37. Cooperative assembly of simian virus 40 T-antigen hexamers on functional halves of the replication origin.
    J Virol. 1991 Jun;65(6):2798-806 PMID: 1851855
  38. Simian virus 40 T-antigen DNA helicase is a hexamer which forms a binary complex during bidirectional unwinding from the viral origin of DNA replication.
    J Virol. 1992 Feb;66(2):804-15 PMID: 1309914
  39. Nuclease activity of 1,10-phenanthroline-copper in study of protein-DNA interactions.
    Methods Enzymol. 1991;208:414-33 PMID: 1779842
  40. Interaction of DNA polymerase alpha-primase with cellular replication protein A and SV40 T antigen.
    EMBO J. 1992 Feb;11(2):769-76 PMID: 1311258
  41. ATP-dependent recognition of eukaryotic origins of DNA replication by a multiprotein complex.
    Nature. 1992 May 14;357(6374):128-34 PMID: 1579162
  42. Protein-DNA interactions at a yeast replication origin.
    Nature. 1992 May 14;357(6374):169-72 PMID: 1579168
  43. Strand-specific recognition of a synthetic DNA replication fork by the SV40 large tumor antigen.
    Science. 1992 Jun 19;256(5064):1656-61 PMID: 1319087
  44. Binding properties of replication protein A from human and yeast cells.
    Mol Cell Biol. 1992 Jul;12(7):3050-9 PMID: 1320195
  45. The simian virus 40 T antigen double hexamer assembles around the DNA at the replication origin.
    J Biol Chem. 1992 Jul 15;267(20):14129-37 PMID: 1321135
  46. Structure and function of simian virus 40 large tumor antigen.
    Annu Rev Biochem. 1992;61:55-85 PMID: 1323237
  47. Inhibition of structural changes in the simian virus 40 core origin of replication by mutation of essential origin sequences.
    J Virol. 1992 Sep;66(9):5248-55 PMID: 1323692
  48. Essential contact residues within SV40 large T antigen binding sites I and II identified by alkylation-interference.
    Cell. 1984 Jan;36(1):155-62 PMID: 6319005
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-02-15
Pages
982-92
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394900
Subset
IM
Grants
NIAID NIH HHS · AI29963 · United States
NCI NIH HHS · NCI P30CA16087 · United States
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2