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PMID: 2557626 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Multiple functions of human single-stranded-DNA binding protein in simian virus 40 DNA replication: single-strand stabilization and stimulation of DNA polymerases alpha and delta.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 24 ·1989-12-00 ·Pages 9757-61

Kenny MK, Lee SH, Hurwitz J

Abstract

The human single-stranded-DNA binding protein (human SSB) is required for simian virus 40 (SV40) DNA replication in vitro. SV40 large tumor antigen and human SSB can support extensive unwinding of SV40 origin-containing DNA in the presence of ATP and a topoisomerase that relieves positive superhelicity. Although SSBs from viral and prokaryotic sources substituted for human SSB in the DNA-unwinding reaction, they did not substitute in the replication of SV40 DNA. The specificity for human SSB in SV40 DNA replication can be explained, at least in part, by the finding that DNA polymerase alpha was stimulated 10-fold by human SSB but not by other SSBs. Human SSB also stimulated proliferating-cell nuclear antigen-dependent DNA polymerase delta; however, other SSBs stimulated this polymerase as well.

MeSH Terms
DNA Polymerase II/metabolism DNA Polymerase III DNA Replication DNA, Single-Stranded/metabolism DNA, Viral/genetics,metabolism DNA-Binding Proteins/metabolism,pharmacology DNA-Directed DNA Polymerase/metabolism Enzyme Activation HeLa Cells/enzymology Humans Kinetics Simian virus 40/genetics
Chemicals
DNA, Single-Stranded DNA, Viral DNA-Binding Proteins DNA Polymerase II DNA Polymerase III DNA-Directed DNA Polymerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Kenny M K
Graduate Program in Molecular Biology, Sloan-Kettering Cancer Center, New York, NY 10021.
Lee S H
Hurwitz J
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53 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.24.9757
Published
1989-12-00
Pages
9757-61
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC298580
Subset
IM
Grants
NIGMS NIH HHS · GM34559 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.24.9757 PMC Full Text View journal details
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