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PMID: 2567992 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Studies on the DNA elongation inhibitor and its proliferating cell nuclear antigen-dependent control in simian virus 40 DNA replication in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 86 ·No. 13 ·1989-07-00 ·Pages 4877-81

Lee SH, Kwong AD, Ishimi Y, Hurwitz J

Abstract

A 120-kDa protein that blocks DNA termini has been purified from extracts of HeLa cells. This protein inhibits the action of a number of enzymes that catalyze reactions involving the 5' and 3' ends of DNA (DNA ligase, 3' and 5' exonucleases, and DNA polymerase alpha). The 120-kDa protein blocks the synthesis of long DNA chains that are normally formed during simian virus 40 DNA replication, causing the accumulation of small DNA fragments. The effects of this protein can be reversed by the addition of proliferating cell nuclear antigen and other protein fractions (activators).

MeSH Terms
Antigens, Neoplasm DNA Ligases/metabolism DNA Polymerase II/metabolism DNA Replication DNA, Viral/antagonists & inhibitors,biosynthesis DNA-Binding Proteins/isolation & purification,metabolism Exodeoxyribonuclease V Exodeoxyribonucleases/metabolism HeLa Cells/metabolism Humans Kinetics Molecular Weight Nuclear Proteins/physiology Proliferating Cell Nuclear Antigen Simian virus 40/metabolism
Chemicals
Antigens, Neoplasm DNA, Viral DNA-Binding Proteins Nuclear Proteins Proliferating Cell Nuclear Antigen DNA Polymerase II Exodeoxyribonucleases Exodeoxyribonuclease V DNA Ligases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lee S H
Graduate Program in Molecular Biology, Memorial Sloan-Kettering Cancer Center, New York, NY 10021.
Kwong A D
Ishimi Y
Hurwitz J
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19 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.86.13.4877
Published
1989-07-00
Pages
4877-81
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC297518
Subset
IM
Grants
NIGMS NIH HHS · GM-34559 · United States
External Links
PubMed source DOI: 10.1073/pnas.86.13.4877 PMC Full Text View journal details
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