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PMID: 1311258 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Interaction of DNA polymerase alpha-primase with cellular replication protein A and SV40 T antigen.

The EMBO journal ·Vol. 11 ·No. 2 ·1992-02-00 ·Pages 769-76

Dornreiter I, Erdile LF, Gilbert IU, von Winkler D, Kelly TJ, Fanning E

Abstract

The purified human single-stranded DNA binding protein, replication protein A (RP-A), forms specific complexes with purified SV40 large T antigen and with purified DNA polymerase alpha-primase, as shown by ELISA and a modified immunoblotting technique. RP-A associated efficiently with the isolated primase, as well as with intact polymerase alpha-primase. The 70 kDa subunit of RP-A was sufficient for association with polymerase alpha-primase. Purified SV40 large T antigen bound to intact RP-A and to polymerase-primase, but not to any of the separated subunits of RP-A or to the isolated primase. These results suggest that the specific protein-protein interactions between RP-A, polymerase-primase and T antigen may play a role in the initiating of SV40 DNA replication.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal Antigens, Polyomavirus Transforming/metabolism Cattle DNA Primase DNA-Binding Proteins/metabolism Enzyme-Linked Immunosorbent Assay Escherichia coli/metabolism Immunoblotting Kinetics Macromolecular Substances Models, Structural Molecular Sequence Data Protein Binding Protein Conformation RNA Nucleotidyltransferases/isolation & purification,metabolism Replication Protein A Simian virus 40/metabolism Thymus Gland/enzymology
Chemicals
Antibodies, Monoclonal Antigens, Polyomavirus Transforming DNA-Binding Proteins Macromolecular Substances Replication Protein A DNA Primase RNA Nucleotidyltransferases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Dornreiter I
Institute for Biochemistry, Munich, Germany.
Erdile L F
Gilbert I U
von Winkler D
Kelly T J
Fanning E
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1992-02-00
Pages
769-76
Language
English
Region
England
NLM ID
8208664
PMCID
PMC556510
Subset
IM
Grants
NIGMS NIH HHS · GM 42780 · United States
External Links
PubMed source PMC Full Text View journal details
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