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PMID: 6258025 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Restriction and modification in Bacillus subtilis: identification of a gene in the temperate phage SP beta coding for a BsuR specific modification methyltransferase.

Molecular & general genetics : MGG ·Vol. 180 ·No. 2 ·1980-00-00 ·Pages 361-7

Trautner TA, Pawlek B, Günthert U, Canosi U, Jentsch S, Freund M

Abstract

A gene coding for a modifying DNA-methyltransferase which methylates the central C in the BsuR recognition sequence 5'GGCC was identified in the genome of the temperature Bacillus subtilis phage SP beta. This gene is expressed only after induction of the prophage by either mitomycin C or UV. The presence of active methyltransferase in induced cells leads to modification of BsuR recognition sites in SP beta DNA as well as in heterologous DNA.

MeSH Terms
Bacillus subtilis/genetics Bacteriophages/enzymology,genetics,radiation effects Base Sequence DNA (Cytosine-5-)-Methyltransferases/genetics DNA Restriction Enzymes/metabolism DNA, Viral/metabolism DNA-Cytosine Methylases Deoxyribonucleases, Type II Site-Specific Genes, Viral Lysogeny Methyltransferases/genetics Mutation Ultraviolet Rays Virus Activation
Chemicals
DNA, Viral DNA modification methylase BsuR DNA-Cytosine Methylases Methyltransferases DNA (Cytosine-5-)-Methyltransferases DNA Restriction Enzymes Deoxyribonucleases, Type II Site-Specific GCAGC-specific type II deoxyribonucleases
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Trautner T A
Pawlek B
Günthert U
Canosi U
Jentsch S
Freund M
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41 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00425849
Published
1980-00-00
Pages
361-7
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00425849
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