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PMID: 329108 Published · ppublish English Journal Article

Active protection by bacteriophages T3 and T7 against E. coli B- and K-specific restriction of their DNA.

Molecular & general genetics : MGG ·Vol. 153 ·No. 1 ·1977-05-20 ·Pages 99-106

Krüger DH, Schroeder C, Hansen S, Rosenthal HA

Abstract

The bacteriophages T3 and T7 are not modified and restricted by E. coli strains with different host specificity (E. coli B, K, O) in vivo. The phages code for a gene product with the ability to overcome classical restriction (ocr): ocr- mutants are subject to modification and restriction via DNA methylation vs cleavage. The T3 genome possesses recognition sites for the restriction endonuclease R.EcoB which, unless the DNA is B-specifically modified, trigger 5-7 DNA cleavages. The ocr gene function of T3 and T7 is located within the gene 0.3 region of these phages and is not identical with the sam (SAMase) function of T3. The mechanism of ocr protection remains unclear, while it is certain that this protection by the gene 0.3 protein is exerted in the infected cell and not through "over-all" modification in the preceding growth cycle of the phage.

MeSH Terms
Adenosylmethionine Decarboxylase/metabolism Coliphages DNA Restriction Enzymes/metabolism DNA Viruses DNA, Viral Endonucleases/metabolism Escherichia coli/metabolism Genes Mutation Phenotype
Chemicals
DNA, Viral Endonucleases DNA Restriction Enzymes Adenosylmethionine Decarboxylase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Krüger D H
Schroeder C
Hansen S
Rosenthal H A
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40 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF01036001
Published
1977-05-20
Pages
99-106
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01036001
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