Unusual behaviour of SPO1 DNA with respect to restriction and modification enzymes recognizing the sequence 5'-G-G-C-C.

Molecular & general genetics : MGG ·Vol. 178 ·No. 1 ·1980-04-00 ·Pages 229-31

Reeve JN, Amann E, Tailor R, Günthert U, Scholz K, Trautner TA

Abstract

SPO1 DNA contains only 5 cleavage sites for restriction enzymes which recognize and cleave the sequence 5'-G-G-C-C (HaeIII or BsuR). Fragments of SPO1 DNA cloned in E. coli to substitute 5'-hydroxymethyluracil (HMU) by thymine (T) remain resistant to HaeIII indicating that this unexpectedly small number of cleavages by HaeIII is not correlated with the presence of HMU in the normal phage DNA. It was previously shown that SPO1 is neither subject to B. subtilis R restriction (Trautner et al., 1974) nor modification in vivo (Günthert et al., 1975). We now show that SPO1 DNA can however be restricted and modified in vitro.

MeSH Terms

Bacteriophage lambda/genetics Base Sequence DNA Restriction Enzymes/metabolism DNA, Bacterial/genetics,metabolism DNA, Recombinant/metabolism DNA, Viral/genetics Substrate Specificity

Chemicals

DNA, Bacterial DNA, Recombinant DNA, Viral DNA Restriction Enzymes

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Reeve J N

Amann E

Tailor R

Günthert U

Scholz K

Trautner T A

References (16)

16 references, click to expand

The genome of Bacillus subtilis phage SPP1: the arrangement of restriction endonuclease generated fragments.
Mol Gen Genet. 1979 Jan 10;168(2):165-72 PMID: 109739
Unusual base sequence arrangement in phage phi 29 DNA.
Gene. 1979 Jan;5(1):1-7 PMID: 107059
The bases of the nucleic acids of some bacterial and animal viruses: the occurrence of 5-hydroxymethylcytosine.
Biochem J. 1953 Dec;55(5):774-82 PMID: 13115372
Restriction and modification in B. subtilis. The biochemical basis of modification against endo R. Bsu R restriction.
Mol Gen Genet. 1975 Dec 30;142(3):185-91 PMID: 815782
Specificity of substrate recognition by the EcoRI restriction endonuclease.
Proc Natl Acad Sci U S A. 1975 Sep;72(9):3310-4 PMID: 242001
Restriction and modification in B. subtilis. Nucleotide sequence recognised by restriction endonuclease R. Bsu R from strain R.
Mol Gen Genet. 1975 Dec 30;143(1):25-33 PMID: 814403
Bacillus subtilis bacteriophages SP82, SPO1, and phie: a comparison of DNAs and of peptides synthesized during infection.
J Virol. 1978 Sep;27(3):725-37 PMID: 100607
EcoRI cleavage of DNA from Bacillus subtilis phage SPO1.
Virology. 1978 Apr;85(2):601-5 PMID: 96586
The effects of substituted pyrimidines in DNAs on cleavage by sequence-specific endonucleases.
J Biol Chem. 1979 Apr 10;254(7):2551-60 PMID: 372183
DNA methylation in adenovirus, adenovirus-transformed cells, and host cells.
Proc Natl Acad Sci U S A. 1976 Nov;73(11):3923-7 PMID: 1069277
Restriction and modification enzymes and their recognition sequences.
Gene. 1978 Nov;4(3):183-94 PMID: 369952
Lambdoid phages that simplify the recovery of in vitro recombinants.
Mol Gen Genet. 1977 Jan 7;150(1):53-61 PMID: 319344
Restriction and modification in Bacillus subtilis. Localization of the methylated nucleotide in the BsuRI recognition sequence.
Eur J Biochem. 1978 Oct 16;90(3):581-3 PMID: 213280
EcoRI cleavage and methylation of DNAs containing modified pyrimidines in the recogintion sequence.
J Biol Chem. 1977 May 25;252(10):3185-93 PMID: 863878
Restriction and modification in B. subtilis. Biological aspects.
Mol Gen Genet. 1974;131(3):181-91 PMID: 4215951
Bacteriophages of Bacillus subtilis.
Bacteriol Rev. 1975 Sep;39(3):257-315 PMID: 809034

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00267234

Published

1980-04-00

Pages

229-31

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00267234

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Unusual behaviour of SPO1 DNA with respect to restriction and modification enzymes recognizing the sequence 5'-G-G-C-C.

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