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PMID: 6091108 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Processing of complex heteroduplexes in Escherichia coli and Cos-1 monkey cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 18 ·1984-09-00 ·Pages 5792-6

Abastado JP, Cami B, Dinh TH, Igolen J, Kourilsky P

Abstract

Upon transformation into Escherichia coli or Cos-1 monkey cells, heteroduplex DNA made of two sequences containing many nucleotide mismatches yields a wide array of different molecules, some with a patchwork structure. Thus, complex heteroduplexes can be processed to generate many genetic variants.

MeSH Terms
Animals Base Sequence Cell Line Chlorocebus aethiops DNA/analysis,genetics DNA Restriction Enzymes DNA, Bacterial/genetics Escherichia coli/genetics Nucleic Acid Heteroduplexes Nucleic Acid Hybridization Plasmids
Chemicals
DNA, Bacterial Nucleic Acid Heteroduplexes DNA DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Abastado J P
Cami B
Dinh T H
Igolen J
Kourilsky P
References (38)
38 references, click to expand
  1. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  2. A general model for genetic recombination.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):358-61 PMID: 1054510
  3. Mismatch repair in heteroduplex DNA.
    Proc Natl Acad Sci U S A. 1975 Jun;72(6):2202-6 PMID: 1094458
  4. A map of temperature-sensitive mutants of simian virus 40.
    Virology. 1975 Jul;66(1):70-81 PMID: 166506
  5. Fate of mismatched base-pair regions in polyoma heteroduplex DNA during infection of mouse cells.
    Proc Natl Acad Sci U S A. 1976 Sep;73(9):3073-7 PMID: 184457
  6. Repair tracts in mismatched DNA heteroduplexes.
    Proc Natl Acad Sci U S A. 1976 Nov;73(11):4135-9 PMID: 1069303
  7. Lambdoid phages that simplify the recovery of in vitro recombinants.
    Mol Gen Genet. 1977 Jan 7;150(1):53-61 PMID: 319344
  8. The frameshift mutability of polA1 and recA1 derivatives of mutator strains of Escherichia coli.
    Mutat Res. 1977 Mar;42(3):443-6 PMID: 323701
  9. Genetic recombination: strand transfer and mismatch repair.
    Annu Rev Biochem. 1978;47:847-80 PMID: 150254
  10. Insertion of a rabbit beta-globin gene sequence into an E. coli plasmid.
    Nucleic Acids Res. 1975 Dec;2(12):2365-78 PMID: 802513
  11. Antibody diversity.
    Science. 1978 Oct 6;202(4363):11-7 PMID: 99815
  12. DNA-mediated transfer of the adenine phosphoribosyltransferase locus into mammalian cells.
    Proc Natl Acad Sci U S A. 1979 Mar;76(3):1373-6 PMID: 286319
  13. Escherichia coli mutator mutants deficient in methylation-instructed DNA mismatch correction.
    Proc Natl Acad Sci U S A. 1980 Feb;77(2):1063-7 PMID: 6987663
  14. Isolation of mutants of an animal virus in bacteria.
    Science. 1980 Sep 19;209(4463):1392-6 PMID: 6251547
  15. Human fetal G gamma- and A gamma-globin genes: complete nucleotide sequences suggest that DNA can be exchanged between these duplicated genes.
    Cell. 1980 Oct;21(3):627-38 PMID: 7438203
  16. SV40-transformed simian cells support the replication of early SV40 mutants.
    Cell. 1981 Jan;23(1):175-82 PMID: 6260373
  17. Gene conversion: some implications for immunoglobulin genes.
    Cell. 1981 Jun;24(3):592-4 PMID: 7018693
  18. Inhibition of SV40 replication in simian cells by specific pBR322 DNA sequences.
    Nature. 1981 Sep 3;293(5827):79-81 PMID: 6267479
  19. Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin.
    Proc Natl Acad Sci U S A. 1981 Nov;78(11):6613-7 PMID: 6171820
  20. Identification of an H-2Kb-related molecule by molecular cloning.
    Immunogenetics. 1981;14(5):383-92 PMID: 7333658
  21. Comparison of nucleotide sequences of mRNAs belonging to the mouse H-2 multigene family.
    Nucleic Acids Res. 1982 Feb 11;10(3):1039-49 PMID: 6278432
  22. Conservation and diversification of genes by mismatch correction and SOS induction.
    Biochimie. 1982 Aug-Sep;64(8-9):559-64 PMID: 6814501
  23. Gene conversion between repeated genes.
    Nature. 1982 Nov 18;300(5889):216-7 PMID: 6755261
  24. Spontaneous H-2 mutants provide evidence that a copy mechanism analogous to gene conversion generates polymorphism in the major histocompatibility complex.
    Proc Natl Acad Sci U S A. 1983 Jan;80(1):242-6 PMID: 6571997
  25. Homologous pairing and strand exchange in genetic recombination.
    Annu Rev Genet. 1982;16:405-37 PMID: 6297377
  26. Gene conversion and polymorphism: generation of mouse immunoglobulin gamma 2a chain alleles by differential gene conversion by gamma 2b chain gene.
    Cell. 1983 Feb;32(2):515-23 PMID: 6297797
  27. The structure of a mutant H-2 gene suggests that the generation of polymorphism in H-2 genes may occur by gene conversion-like events.
    Nature. 1983 Feb 24;301(5902):671-4 PMID: 6828150
  28. Effects of nonhomology on bacteriophage lambda recombination.
    Genetics. 1983 Jan;103(1):5-22 PMID: 6220942
  29. Genetic exchanges between partially homologous nucleotide sequences: possible implications for multigene families.
    Biochimie. 1983 Feb;65(2):85-93 PMID: 6405807
  30. Rearrangement and mutagenesis of a shuttle vector plasmid after passage in mammalian cells.
    Proc Natl Acad Sci U S A. 1983 May;80(10):3010-4 PMID: 6304690
  31. High mutation frequency in DNA transfected into mammalian cells.
    Proc Natl Acad Sci U S A. 1983 May;80(10):3015-9 PMID: 6574469
  32. Gene conversion as a mechanism for antigenic variation in trypanosomes.
    Cell. 1983 Sep;34(2):371-81 PMID: 6616615
  33. Effects of high levels of DNA adenine methylation on methyl-directed mismatch repair in Escherichia coli.
    Genetics. 1983 Aug;104(4):571-82 PMID: 6225697
  34. Gene conversion and associated reciprocal recombination are separable events in vegetative cells of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1983 Nov;80(22):6912-6 PMID: 6359159
  35. Correction of complex heteroduplexes made of mouse H-2 gene sequences in Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1984 Jan;81(2):503-7 PMID: 6320192
  36. Gene conversion: point-mutation heterozygosities lower heteroduplex formation.
    EMBO J. 1983;2(12):2265-70 PMID: 6667676
  37. Human fetal globin DNA sequences suggest novel conversion event.
    Nucleic Acids Res. 1984 Jun 11;12(11):4469-79 PMID: 6330670
  38. Genes of the major histocompatibility complex of the mouse.
    Annu Rev Immunol. 1983;1:529-68 PMID: 6152713
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.18.5792
Published
1984-09-00
Pages
5792-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC391797
Subset
IM
Databases
GENBANK
K02753
External Links
PubMed source DOI: 10.1073/pnas.81.18.5792 PMC Full Text View journal details
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