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PMID: 6171820 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Use of synthetic oligonucleotides as hybridization probes: isolation of cloned cDNA sequences for human beta 2-microglobulin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 78 ·No. 11 ·1981-11-00 ·Pages 6613-7

Suggs SV, Wallace RB, Hirose T, Kawashima EH, Itakura K

Abstract

We have synthesized two sets of 15-base-long oligodeoxyribonucleotides corresponding to all possible coding sequences for a small portion of human beta 2-microglobulin. Labeled oligonucleotides were used as hybridization probes to screen bacterial clones containing cDNA sequences primed with oligo(dT) and inserted into the plasmid vector pBR322. One beta 2-microglobulin cDNA clone was detected in the 535 bacterial plasmid clones that were screened. The clone has been characterized by blotting and nucleotide sequence analysis. The cloned beta 2-microglobulin sequence contains 217 base pairs of the 3' untranslated region of the mRNA and 328 base pairs (97%) of the coding region.

MeSH Terms
Amino Acid Sequence Base Sequence Beta-Globulins/genetics Cloning, Molecular DNA, Recombinant/isolation & purification Escherichia coli/genetics Humans Nucleic Acid Hybridization Oligodeoxyribonucleotides/chemical synthesis Oligonucleotides/chemical synthesis Plasmids beta 2-Microglobulin/genetics
Chemicals
Beta-Globulins DNA, Recombinant Oligodeoxyribonucleotides Oligonucleotides beta 2-Microglobulin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Suggs S V
Wallace R B
Hirose T
Kawashima E H
Itakura K
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35 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.78.11.6613
Published
1981-11-00
Pages
6613-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349099
Subset
IM
Grants
NIGMS NIH HHS · GM07591 · United States
NIGMS NIH HHS · GM25658 · United States
NIGMS NIH HHS · GM26391 · United States
Databases
GENBANK
J00105
External Links
PubMed source DOI: 10.1073/pnas.78.11.6613 PMC Full Text View journal details
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