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PMID: 6330670 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Human fetal globin DNA sequences suggest novel conversion event.

Nucleic acids research ·Vol. 12 ·No. 11 ·1984-06-11 ·Pages 4469-79

Stoeckert CJ, Collins FS, Weissman SM

Abstract

DNA sequencing studies of two recently cloned human A gamma globin alleles has revealed a number of base differences which are clustered in the large intron (IVS-2). One allele has a previously undescribed IVS-2 sequence. Most of the allelic differences can be explained as resulting from a gene conversion event involving G gamma as a donor. A novel feature of this event is that three G gamma-like regions occur interspersed among unconverted areas of the A gamma gene. We propose that an alternating purine-pyrimidine run which is located between two of the converted sites is the initiation site of the conversion event. Consistent with models of gene conversion, this poly (purine-pyrimidine) tract has single-stranded characteristics in supercoiled plasmids as assayed by S1-nuclease.

MeSH Terms
Alleles Base Sequence Cloning, Molecular Endonucleases Female Fetal Proteins/genetics Fetus Genes Globins/genetics Humans Leukocytes/analysis Plasmids Pregnancy Single-Strand Specific DNA and RNA Endonucleases
Chemicals
Fetal Proteins Globins Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stoeckert C J
Collins F S
Weissman S M
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35 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.11.4469
Published
1984-06-11
Pages
4469-79
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318851
Subset
IM
Grants
NCI NIH HHS · 5T32CA0959-08 · United States
NIGMS NIH HHS · GM-32156 · United States
Databases
GENBANK
J00093, J00094, J00096, J00158, J00159, J00160, J00161, J00162, J00163, J00164, J00165, J00166, J00167, J00168, J00169, J00170, J00171, J00172, J00173, J00174, J00175, J00177, J00178, J00179, K01239, K01890, K02544, M18047, M19067, X00423
External Links
PubMed source DOI: 10.1093/nar/12.11.4469 PMC Full Text View journal details
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