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PMID: 6304690 Published · ppublish English Journal Article

Rearrangement and mutagenesis of a shuttle vector plasmid after passage in mammalian cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 80 ·No. 10 ·1983-05-00 ·Pages 3010-4

Razzaque A, Mizusawa H, Seidman MM

Abstract

A shuttle vector plasmid that contains sequences from simian virus 40, pBR322, and a bacterial marker gene, galactokinase, has been constructed. After replication in cells permissive for virus progeny, plasmid DNA was introduced into a galactokinase-deficient bacterial strain and the relative frequency of colonies with plasmids but without galactokinase activity was determined. This assay showed that 1% of the plasmids were defective after passage in the mammalian cells. Individual mutant plasmids were examined and found to contain deletions, duplications, point mutations, and insertions of cell DNA.

MeSH Terms
Animals Cells, Cultured Chlorocebus aethiops Chromosome Deletion DNA Replication DNA Transposable Elements Genetic Vectors Molecular Weight Mutation Plasmids
Chemicals
DNA Transposable Elements
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Razzaque A
Mizusawa H
Seidman M M
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33 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.80.10.3010
Published
1983-05-00
Pages
3010-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC393963
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.80.10.3010 PMC Full Text View journal details
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