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PMID: 6259618 Published · ppublish English Journal Article

Sequence relationships between single repeat units of highly reiterated African Green monkey DNA.

Nucleic acids research ·Vol. 9 ·No. 1 ·1981-01-10 ·Pages 169-81

Thayer RE, Singer MF, McCutchan TF

Abstract

Individual monomer and dimer units of the highly repeated alpha-component DNA of African Green monkeys were isolated and amplified by molecular cloning in pBR322. The purified sequences were characterized by digestion with restriction endonucleases and by primary nucleotide sequence analysis. Comparison of the cloned units with the 172 base pair long sequence representing the most abundant nucleotide at each position in the set of sequences comprising alpha-component allows the following conclusions. The set of sequences comprising alpha-component is made up of a very large number of related but slightly divergent sequences. Two neighboring repeats of the monomer unit are not necessarily more similar to one another than are randomly isolated monomers.

MeSH Terms
Animals Chlorocebus aethiops Cloning, Molecular DNA DNA Restriction Enzymes DNA, Recombinant Repetitive Sequences, Nucleic Acid
Chemicals
DNA, Recombinant DNA DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Thayer R E
Singer M F
McCutchan T F
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19 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.1.169
Published
1981-01-10
Pages
169-81
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326676
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.1.169 PMC Full Text View journal details
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