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PMID: 1069303 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Repair tracts in mismatched DNA heteroduplexes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 73 ·No. 11 ·1976-11-00 ·Pages 4135-9

Wagner R, Meselson M

Abstract

Heteroduplexes with mismatches at four sites were constructed from separated strands of lambda DNA and used to transfect Escherichia coli under recombinationless conditions. The output phages from 967 single cells in one experiment and 1016 in another were analyzed to determine the pattern of mismatch repair. A wide range of repair frequencies was found among the mismatches studied. Repair involving two or more close sites in the same heteroduplex occurs much more often on thesamestrandthanonopposite strands. Analysis of the pattern of repair suggeststhat repair tracts initiate at mismatches, propagate preferentially in the 5'leads to 3' direction, and extend an average distance of ca 3000 nucleotides.

MeSH Terms
Base Sequence Chromosome Mapping Coliphages/metabolism DNA Repair DNA, Viral/metabolism Genotype Molecular Weight
Chemicals
DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wagner R
Meselson M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.73.11.4135
Published
1976-11-00
Pages
4135-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC431357
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.73.11.4135 PMC Full Text View journal details
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