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PMID: 3697078 Published · ppublish English Journal Article

Putative polyadenylation signals in nuclear genes of higher plants: a compilation and analysis.

Nucleic acids research ·Vol. 15 ·No. 23 ·1987-12-10 ·Pages 9627-40

Joshi CP

Abstract

In animal and viral pre-mRNAS, the process of polyadenylation is mediated through several cis-acting poly (A) signals present upstream and downstream from poly (A) sites. The situation regarding polyadenylation of higher plant pre-mRNAS, however, has remained obscure so far. In this paper, a search for putative poly (A) signals is made by considering the published data from 46 plant genomic DNA sequences. Certain domains in the 3' untranslated regions from nuclear genes of higher plants were compiled and occurrence of sequence motifs such as AATAAA, CAYTG, YGTGTTYY and YAYTG was scored in relation to poly (A) sites. Moreover, consensus sequences for important regions in the 3' untranslated sequences and poly (A) signals were also deduced from the data. It was inferred that sequence motifs similar to poly (A) signals exist around poly (A) sites but some of them are in entirely different spatial relationship than observed in other eukaryotes. This indicates their probable non-involvement in the process of polyadenylation in higher plants necessitating a functional analysis approach to define the plant specific poly (A) signals.

MeSH Terms
Animals Base Sequence Codon DNA/genetics Genes Plants/genetics Poly A/genetics Protein Biosynthesis
Chemicals
Codon Poly A DNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Joshi C P
Division of Biochemical Sciences, National Chemical Laboratory, Pune, India.
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.23.9627
Published
1987-12-10
Pages
9627-40
Language
English
Region
England
NLM ID
0411011
PMCID
PMC306520
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/15.23.9627 PMC Full Text View journal details
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