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PMID: 3014439 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Requirement of A-A-U-A-A-A and adjacent downstream sequences for SV40 early polyadenylation.

Nucleic acids research ·Vol. 14 ·No. 12 ·1986-06-25 ·Pages 4939-52

Kessler MM, Beckendorf RC, Westhafer MA, Nordstrom JL

Abstract

RNA mapping experiments and chloramphenicol acetyltransferase assays were used to analyze polyadenylation in COS cells of transcripts from derivatives of pSV2-neo and pSV2-cat, in which the SV40 early poly(A) signal has been modified. Neither the sequence A-A-U-A-A-A nor the sequences located immediately downstream from it in the SV40 early gene appear to function by themselves as a poly(A) signal. When combined, however, these two elements form a poly(A) signal whose efficiency and specificity closely resemble those of the wild type signal. The addition of six nucleotides between the A-A-U-A-A-A sequence and the poly(A) site has no detectable effect on the efficiency or site of polyadenylation. Deletion of the 60 nucleotides immediately upstream from the hexanucleotide also has no detectable effect on polyadenylation. Therefore, A-A-U-A-A-A and sequences downstream from it appear to be sufficient for SV40 early polyadenylation.

MeSH Terms
Animals Base Sequence Cell Line Chlorocebus aethiops Chromosome Mapping Cloning, Molecular Endonucleases Gene Expression Regulation Poly A/genetics RNA Processing, Post-Transcriptional RNA, Messenger/genetics RNA, Viral/genetics Simian virus 40/genetics Single-Strand Specific DNA and RNA Endonucleases
Chemicals
RNA, Messenger RNA, Viral Poly A Endonucleases Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kessler M M
Beckendorf R C
Westhafer M A
Nordstrom J L
References (38)
38 references, click to expand
  1. Molecular structure and flanking nucleotide sequences of the natural chicken ovomucoid gene.
    Cell. 1979 Nov;18(3):829-42 PMID: 519756
  2. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  3. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  4. Ribonucleic acid isolated by cesium chloride centrifugation.
    Biochemistry. 1974 Jun 4;13(12):2633-7 PMID: 4831907
  5. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  6. Steps in the processing of Ad2 mRNA: poly(A)+ nuclear sequences are conserved and poly(A) addition precedes splicing.
    Cell. 1978 Dec;15(4):1477-93 PMID: 729004
  7. Simian virus 40 early mRNA's. I. Genomic localization of 3' and 5' termini and two major splices in mRNA from transformed and lytically infected cells.
    J Virol. 1979 Apr;30(1):279-96 PMID: 90157
  8. Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
    Biochemistry. 1979 Nov 27;18(24):5294-9 PMID: 518835
  9. Sequencing end-labeled DNA with base-specific chemical cleavages.
    Methods Enzymol. 1980;65(1):499-560 PMID: 6246368
  10. Transcription maps of polyoma virus-specific RNA: analysis by two-dimensional nuclease S1 gel mapping.
    Methods Enzymol. 1980;65(1):718-49 PMID: 6154876
  11. Two mRNAs can be produced from a single immunoglobulin mu gene by alternative RNA processing pathways.
    Cell. 1980 Jun;20(2):313-9 PMID: 6771020
  12. SV40-transformed simian cells support the replication of early SV40 mutants.
    Cell. 1981 Jan;23(1):175-82 PMID: 6260373
  13. The sequence 5'-AAUAAA-3'forms parts of the recognition site for polyadenylation of late SV40 mRNAs.
    Cell. 1981 Apr;24(1):251-60 PMID: 6113054
  14. Complete nucleotide sequence of the chicken chromosomal ovalbumin gene and its biological significance.
    Biochemistry. 1981 Oct 27;20(22):6437-46 PMID: 6272839
  15. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  16. Recombinant genomes which express chloramphenicol acetyltransferase in mammalian cells.
    Mol Cell Biol. 1982 Sep;2(9):1044-51 PMID: 6960240
  17. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  18. High efficiency polyoma DNA transfection of chloroquine treated cells.
    Nucleic Acids Res. 1983 Mar 11;11(5):1295-308 PMID: 6298741
  19. Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3' consensus sequence AAUAAA.
    Nature. 1983 Oct 13-19;305(5935):600-5 PMID: 6194440
  20. Alpha-thalassaemia caused by a polyadenylation signal mutation.
    Nature. 1983 Nov 24-30;306(5941):398-400 PMID: 6646217
  21. Analysis of processing and polyadenylation signals of the hepatitis B virus surface antigen gene by using simian virus 40-hepatitis B virus chimeric plasmids.
    Mol Cell Biol. 1983 Dec;3(12):2250-8 PMID: 6318092
  22. Are U4 small nuclear ribonucleoproteins involved in polyadenylation?
    Nature. 1984 May 10-16;309(5964):179-82 PMID: 6325940
  23. Requirement for the 3' flanking region of the bovine growth hormone gene for accurate polyadenylylation.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):3944-8 PMID: 6146135
  24. Requirement of a downstream sequence for generation of a poly(A) addition site.
    Cell. 1984 Jul;37(3):993-9 PMID: 6744418
  25. Sequences on the 3' side of hexanucleotide AAUAAA affect efficiency of cleavage at the polyadenylation site.
    Mol Cell Biol. 1984 Aug;4(8):1460-8 PMID: 6149460
  26. Role of the conserved AAUAAA sequence: four AAUAAA point mutants prevent messenger RNA 3' end formation.
    Science. 1984 Nov 30;226(4678):1045-51 PMID: 6208611
  27. A sequence downstream of AAUAAA is required for rabbit beta-globin mRNA 3'-end formation.
    Nature. 1984 Nov 29-Dec 5;312(5993):473-4 PMID: 6095108
  28. RNA sequence containing hexanucleotide AAUAAA directs efficient mRNA polyadenylation in vitro.
    Mol Cell Biol. 1985 Feb;5(2):373-9 PMID: 2579321
  29. Transcription termination and 3' processing: the end is in site!
    Cell. 1985 Jun;41(2):349-59 PMID: 2580642
  30. The consensus sequence YGTGTTYY located downstream from the AATAAA signal is required for efficient formation of mRNA 3' termini.
    Nucleic Acids Res. 1985 Feb 25;13(4):1347-68 PMID: 2987822
  31. A sequence downstream of A-A-U-A-A-A is required for formation of simian virus 40 late mRNA 3' termini in frog oocytes.
    Proc Natl Acad Sci U S A. 1985 Jun;82(12):3949-53 PMID: 2987956
  32. Thalassemia due to a mutation in the cleavage-polyadenylation signal of the human beta-globin gene.
    EMBO J. 1985 Feb;4(2):453-6 PMID: 4018033
  33. Poly(A) site cleavage in a HeLa nuclear extract is dependent on downstream sequences.
    Cell. 1985 Dec;43(3 Pt 2):677-83 PMID: 2866847
  34. Role of an RNA cleavage/poly(A) addition site in the production of membrane-bound and secreted IgM mRNA.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8658-62 PMID: 3936040
  35. Identification of a sequence element on the 3' side of AAUAAA which is necessary for simian virus 40 late mRNA 3'-end processing.
    Mol Cell Biol. 1985 Oct;5(10):2713-9 PMID: 3016512
  36. Definition of essential sequences and functional equivalence of elements downstream of the adenovirus E2A and the early simian virus 40 polyadenylation sites.
    Mol Cell Biol. 1985 Nov;5(11):2975-83 PMID: 3018490
  37. ATTAAA as well as downstream sequences are required for RNA 3'-end formation in the E3 complex transcription unit of adenovirus.
    Mol Cell Biol. 1985 Nov;5(11):3183-93 PMID: 3018506
  38. The ovalbumin gene-sequence of putative control regions.
    Nucleic Acids Res. 1980 Jan 11;8(1):127-42 PMID: 6243777
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/14.12.4939
Published
1986-06-25
Pages
4939-52
Language
English
Region
England
NLM ID
0411011
PMCID
PMC311502
Subset
IM
Grants
NIGMS NIH HHS · GM 33410 · United States
External Links
PubMed source DOI: 10.1093/nar/14.12.4939 PMC Full Text View journal details
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