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PMID: 6146135 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Requirement for the 3' flanking region of the bovine growth hormone gene for accurate polyadenylylation.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 13 ·1984-07-00 ·Pages 3944-8

Woychik RP, Lyons RH, Post L, Rottman FM

Abstract

We examined whether the sequence extending 3' to the polyadenylylation site of the bovine growth hormone gene contains any signal that affects the polyadenylylation of the growth hormone mRNA. For this purpose, cloned copies of this gene, each containing a different length of growth hormone-specific sequence 3' to the wild-type polyadenylylation site, were used to transfect COS-1 cells. The polyadenylylation site on the mRNAs produced from the exogenously added growth hormone genes were analyzed with an S1 nuclease mapping procedure. We found that a gene containing 84 base pairs of its own 3' flanking sequence is capable of producing an accurately polyadenylylated mRNA. On the other hand, genes containing only 1, 10, or 13 base pairs of 3' flanking sequence were principally polyadenylylated at discrete sites either upstream or downstream from the wild-type position. Using a computer program, we examined whether secondary structures on the primary growth hormone transcript correlated with the site where the mRNA is polyadenylylated.

MeSH Terms
Animals Base Sequence Cattle Cell Line Chlorocebus aethiops DNA Restriction Enzymes Genes Growth Hormone/genetics Kidney Nucleic Acid Conformation Plasmids Poly A/genetics RNA, Messenger/genetics Transfection
Chemicals
RNA, Messenger Poly A Growth Hormone DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Woychik R P
Lyons R H
Post L
Rottman F M
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25 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.13.3944
Published
1984-07-00
Pages
3944-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC345344
Subset
IM
Grants
NIADDK NIH HHS · AM-32770 · United States
NCI NIH HHS · CA-31810 · United States
External Links
PubMed source DOI: 10.1073/pnas.81.13.3944 PMC Full Text View journal details
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