Home LiteratureArticle Details
PMID: 2866954 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A nuclear gene encoding the beta subunit of the mitochondrial ATP synthase in Nicotiana plumbaginifolia.

The EMBO journal ·Vol. 4 ·No. 9 ·1985-09-00 ·Pages 2159-65

Boutry M, Chua NH

Abstract

The beta subunit of the mitochondrial ATP synthase in Nicotiana plumbaginifolia is encoded by two nuclear genes, atp2-1 and atp2-2, which are both expressed. The complete nucleotide sequence of atp2-1 has been determined. It contains eight introns ranging from 88 to 1453 bp. The last intron contains a putative insertion element (Inp), of 812 bp bordered by 35-bp inverted repeats which share an 11-bp homology with Agrobacterium tumefaciens T-DNA borders. Sequences homologous to Inp are present in multiple copies in the N. plumbaginifolia and the N. tabacum genome but not in more distant species. The atp2-1 encoded polypeptide is highly homologous to beta subunits from other ATP synthases but it contains an extension at the N terminus which is probably involved in mitochondrial targeting. A sequence homology between exon 4 of atp2-1 and exon 1 of the human ras genes suggests a common ancestral origin for these exons.

MeSH Terms
Amino Acid Sequence Base Sequence Cell Nucleus/metabolism Genes Macromolecular Substances Mitochondria/enzymology Nucleic Acid Hybridization Plants/enzymology Plants, Toxic Proton-Translocating ATPases/genetics Tobacco/enzymology
Chemicals
Macromolecular Substances Proton-Translocating ATPases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Boutry M
Chua N H
References (48)
48 references, click to expand
  1. Overlap and cotranscription of the genes for the beta and epsilon subunits of tobacco chloroplast ATPase.
    Gene. 1983 Oct;24(2-3):147-55 PMID: 6227526
  2. Lambda replacement vectors carrying polylinker sequences.
    J Mol Biol. 1983 Nov 15;170(4):827-42 PMID: 6315951
  3. Proton atpases: structure and mechanism.
    Annu Rev Biochem. 1983;52:801-24 PMID: 6225377
  4. The alpha-subunit of the maize F(1)-ATPase is synthesised in the mitochondrion.
    EMBO J. 1983;2(10):1783-9 PMID: 16453476
  5. DNA from the A6S/2 crown gall tumor contains scrambled Ti-plasmid sequences near its junctions with plant DNA.
    Cell. 1982 Jul;29(3):1005-14 PMID: 7151163
  6. Intracellular targeting and import of an F1-ATPase beta-subunit-beta-galactosidase hybrid protein into yeast mitochondria.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):3983-7 PMID: 6330727
  7. Intron-exon splice junctions map at protein surfaces.
    Nature. 1982 Sep 9;299(5879):180-2 PMID: 7110339
  8. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  9. Independent loci for the structural genes of the yeast mitochondrial alpha and beta ATPase subunits.
    J Biol Chem. 1984 Mar 10;259(5):2845-9 PMID: 6230353
  10. The alpha subunit of a plant mitochondrial F1-ATPase is translated in mitochondria.
    J Biol Chem. 1983 Jul 25;258(14):8524-6 PMID: 6223032
  11. Inhibition of RNA cleavage but not polyadenylation by a point mutation in mRNA 3' consensus sequence AAUAAA.
    Nature. 1983 Oct 13-19;305(5935):600-5 PMID: 6194440
  12. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  13. Structure and organization of the human Ki-ras proto-oncogene and a related processed pseudogene.
    Nature. 1983 Aug 11-17;304(5926):501-6 PMID: 6308466
  14. Correlation of exons with structural domains in alcohol dehydrogenase.
    EMBO J. 1984 Jun;3(6):1307-10 PMID: 6378620
  15. Intron/exon structure of the chicken pyruvate kinase gene.
    Cell. 1985 Jan;40(1):81-90 PMID: 2981634
  16. Distantly related sequences in the alpha- and beta-subunits of ATP synthase, myosin, kinases and other ATP-requiring enzymes and a common nucleotide binding fold.
    EMBO J. 1982;1(8):945-51 PMID: 6329717
  17. Kilo-sequencing: creation of an ordered nest of asymmetric deletions across a large target sequence carried on phage M13.
    Methods Enzymol. 1983;101:98-122 PMID: 6310345
  18. Efficient isolation of genes by using antibody probes.
    Proc Natl Acad Sci U S A. 1983 Mar;80(5):1194-8 PMID: 6219389
  19. The amino acid sequence of the beta-subunit of ATP synthase from bovine heart mitochondria.
    J Biol Chem. 1983 Mar 10;258(5):3081-9 PMID: 6298222
  20. Structure and function of proton-translocating adenosine triphosphatase (F0F1): biochemical and molecular biological approaches.
    Microbiol Rev. 1983 Sep;47(3):285-312 PMID: 6226867
  21. Structure and activation of the human N-ras gene.
    Cell. 1983 Sep;34(2):581-6 PMID: 6616621
  22. How are proteins imported into mitochondria?
    Cell. 1983 Feb;32(2):316-8 PMID: 6297790
  23. Nucleotide sequence of the genes for beta and epsilon subunits of proton-translocating ATPase from Escherichia coli.
    Biochem Biophys Res Commun. 1982 Apr 29;105(4):1257-64 PMID: 6285901
  24. Homology between human bladder carcinoma oncogene product and mitochondrial ATP-synthase.
    Nature. 1983 Jan 20;301(5897):262-4 PMID: 6296696
  25. Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue.
    Nature. 1983 Mar 3;302(5903):33-7 PMID: 6298635
  26. Genes in S. cerevisiae encoding proteins with domains homologous to the mammalian ras proteins.
    Cell. 1984 Mar;36(3):607-12 PMID: 6365329
  27. Genes encoding actin in higher plants: intron positions are highly conserved but the coding sequences are not.
    J Mol Appl Genet. 1983;2(1):111-26 PMID: 6842118
  28. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  29. Ribonucleic acid isolated by cesium chloride centrifugation.
    Biochemistry. 1974 Jun 4;13(12):2633-7 PMID: 4831907
  30. Nucleotide sequence of the yeast nuclear gene for cytochrome c peroxidase precursor. Functional implications of the pre sequence for protein transport into mitochondria.
    J Biol Chem. 1982 Dec 25;257(24):15054-8 PMID: 6294090
  31. The biosynthesis of the ubiquinol-cytochrome c reductase complex in yeast. DNA sequence analysis of the nuclear gene coding for the 14-kDa subunit.
    Eur J Biochem. 1984 Jan 2;138(1):169-77 PMID: 6319130
  32. pEMBL: a new family of single stranded plasmids.
    Nucleic Acids Res. 1983 Mar 25;11(6):1645-55 PMID: 6300771
  33. Structure of the Ki-ras gene of the human lung carcinoma cell line Calu-1.
    Nature. 1983 Aug 11-17;304(5926):497-500 PMID: 6308465
  34. ras-Related gene sequences identified and isolated from Saccharomyces cerevisiae.
    Nature. 1983 Dec 15-21;306(5944):707-9 PMID: 6318116
  35. Molecular analysis of the alcohol dehydrogenase (Adh1) gene of maize.
    Nucleic Acids Res. 1984 May 11;12(9):3983-4000 PMID: 6328449
  36. The atp operon: nucleotide sequence of the genes for the gamma, beta, and epsilon subunits of Escherichia coli ATP synthase.
    Nucleic Acids Res. 1981 Oct 24;9(20):5287-96 PMID: 6272217
  37. Mitochondrial genes and translation products.
    Annu Rev Biochem. 1979;48:419-41 PMID: 157715
  38. The proton-ATPase of bacteria and mitochondria.
    J Membr Biol. 1983;73(2):105-24 PMID: 6191035
  39. Buffer gradient gels and 35S label as an aid to rapid DNA sequence determination.
    Proc Natl Acad Sci U S A. 1983 Jul;80(13):3963-5 PMID: 6575390
  40. Molecular analysis of the alcohol dehydrogenase 2 (Adh2) gene of maize.
    Nucleic Acids Res. 1985 Feb 11;13(3):727-43 PMID: 2987807
  41. Sequences of the recA gene and protein.
    Proc Natl Acad Sci U S A. 1980 May;77(5):2611-5 PMID: 6930655
  42. Tumor induction by Agrobacterium tumefaciens: analysis of the boundaries of T-DNA.
    J Mol Appl Genet. 1982;1(4):361-70 PMID: 7108407
  43. Cloned DNA sequences complementary to mRNAs encoding precursors to the small subunit of ribulose-1,5-bisphosphate carboxylase and a chlorophyll a/b binding polypeptide.
    Proc Natl Acad Sci U S A. 1981 Dec;78(12):7304-8 PMID: 16593132
  44. Functional homology of mammalian and yeast RAS genes.
    Cell. 1985 Jan;40(1):19-26 PMID: 2981628
  45. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  46. Nuclear genes coding the yeast mitochondrial adenosine triphosphatase complex. Isolation of ATP2 coding the F1-ATPase beta subunit.
    J Biol Chem. 1983 Oct 10;258(19):11465-70 PMID: 6225776
  47. Why genes in pieces?
    Nature. 1978 Feb 9;271(5645):501 PMID: 622185
  48. Structure and expression of a complementary DNA for the nuclear coded precursor of human mitochondrial ornithine transcarbamylase.
    Science. 1984 Jun 8;224(4653):1068-74 PMID: 6372096
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1985-09-00
Pages
2159-65
Language
English
Region
England
NLM ID
8208664
PMCID
PMC554481
Subset
IM
Grants
NIGMS NIH HHS · GM 30726 · United States
FIC NIH HHS · TW03224 · United States
Databases
GENBANK
X02868
External Links
PubMed source PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2