The alpha-subunit of the maize F(1)-ATPase is synthesised in the mitochondrion.

The EMBO journal ·Vol. 2 ·No. 10 ·1983-00-00 ·Pages 1783-9

Hack E, Leaver CJ

Abstract

The F(1)-ATPase complex has been purified from maize (Zea mays L.) mitochondria and shown to consist of five subunits with mol. wts. of 58 000 (alpha), 56 000 (beta), 35 000 (gamma), 22 000 (delta) and 8000 (epsilon). The alpha-subunit co-migrates on one- and two- dimensional isoelectric focussing-SDS polyacrylamide gels with the major polypeptide synthesised by isolated mitochondria. One-dimensional proteolytic peptide mapping and immunoprecipitation confirms that the alpha-subunit is a mitochondrial translation product and therefore presumably encoded in mitochondrial DNA. This contrasts with the situation in animal and fungal cells where all five subunits of the F(1)-ATPase are encoded by the nuclear genome and synthesised on cytosolic ribosomes.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Hack E

Department of Botany, The King's Buildings, University of Edinburgh, Edinburgh EH9 3JH, UK.

Leaver C J

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1983-00-00

Pages

1783-9

Language

English

Region

England

NLM ID

8208664

PMCID

PMC555359

External Links

PubMed source PMC Full Text View journal details

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The alpha-subunit of the maize F(1)-ATPase is synthesised in the mitochondrion.

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