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PMID: 4256870 Published · ppublish English Journal Article

Cathepsin D. Characteristics of immunoinhibition and the confirmation of a role in cartilage breakdown.

The Biochemical journal ·Vol. 123 ·No. 1 ·1971-06-00 ·Pages 1-13

Dingle JT, Barrett AJ, Weston PD

Abstract

1. Antisera were raised against lysosomal cathepsin D of man, chicken and rabbit. 2. The antisera were found to be specific and potent inhibitors of cathepsin D activity. 3. The immunological nature of the inhibition was established. 4. The inhibitory effect was studied by varying pH, antiserum/enzyme ratio, time of incubation, concentration of components and order of mixing, and by using purified antibody and univalent antibody fragments. 5. The specificities of the antisera were examined with respect to other enzymes, isoenzymes of cathepsin D and cathepsin D from different organs. 6. The antisera prevented the action of cathepsin D on isolated proteoglycans and on cartilage. 7. The antisera produced up to 90% inhibition of the autolysis of cartilage from chicks and rabbits, indicating that cathepsin D is the enzyme mainly responsible for the breakdown of proteoglycans in this system.

MeSH Terms
Animals Antibodies/isolation & purification Antibody Specificity Antigen-Antibody Reactions Autolysis/enzymology Cartilage/enzymology Cathepsins/antagonists & inhibitors,metabolism Cattle Chemical Precipitation Chickens Glycoproteins/metabolism Glycosaminoglycans/metabolism Humans Hydrogen-Ion Concentration Immune Sera/pharmacology Immunodiffusion Immunoglobulin G/isolation & purification Isoenzymes Organ Specificity Rabbits Sheep
Chemicals
Antibodies Glycoproteins Glycosaminoglycans Immune Sera Immunoglobulin G Isoenzymes Cathepsins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dingle J T
Barrett A J
Weston P D
References (35)
35 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1230001
Published
1971-06-00
Pages
1-13
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1176894
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1230001 PMC Full Text View journal details
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