Home LiteratureArticle Details
PMID: 128353 Published · ppublish English Journal Article

A simple and rapid method for the preparation of adenosine triphosphatase from submitochondrial particles.

The Biochemical journal ·Vol. 148 ·No. 3 ·1975-06-00 ·Pages 533-7

Beechey RB, Hubbard SA, Linnett PE, Mitchell AD, Munn EA

Abstract

An almost pure form of the bovine heart mitochondrial adenosine triphosphatase (ATPase) is released from the membrane by shaking submitochondrial particles with chloroform. Analyses on polyacrylamide gels and by electron microscopy, and also sensitivity to inhibitors, show that the chloroform-released enzyme is similar to other ATPase preparations from bovine heart mitochondria.

MeSH Terms
Adenosine Triphosphatases/antagonists & inhibitors,isolation & purification Animals Aurovertins/pharmacology Cattle Chloroform Dicyclohexylcarbodiimide/pharmacology Electrophoresis, Polyacrylamide Gel In Vitro Techniques Methods Microscopy, Electron Mitochondria, Muscle/enzymology Myocardium/enzymology Subcellular Fractions/analysis Ultracentrifugation
Chemicals
Aurovertins Dicyclohexylcarbodiimide Chloroform Adenosine Triphosphatases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Beechey R B
Hubbard S A
Linnett P E
Mitchell A D
Munn E A
References (30)
30 references, click to expand
  1. Partial resolution of the enzymes catalyzing oxidative phosphorylation. VI. Studies on the mechanism of cold inactivation of mitochondrial adenosine triphosphatase.
    J Biol Chem. 1965 Dec;240(12):4694-702 PMID: 4221250
  2. Studies on oxidative phosphorylation. XV. Latent adenosine 5'-triphosphatase activity of factor A.
    Arch Biochem Biophys. 1968 Feb;123(2):385-96 PMID: 4230614
  3. The subunit structure of beef heart mitochondrial adenosine triphosphatase. Isolation procedures.
    J Biol Chem. 1972 Oct 25;247(20):6617-23 PMID: 4263201
  4. The subunit structure of beef heart mitochondrial adenosine triphosphatase. Physical and chemical properties of isolated subunits.
    J Biol Chem. 1972 Oct 25;247(20):6624-30 PMID: 4263202
  5. Partial resolution of the enzymes catalyzing oxidative phosphorylation. XVI. Chemical modification of mitochondrial adenosine triphosphatase.
    J Biol Chem. 1967 Dec 25;242(24):5789-95 PMID: 4319672
  6. Antibiotics as tools for metabolic studies. X. Inhibition of phosphoryl transfer reactions in mitochondria by peliomycin, ossamycin, and venturicidin.
    J Biol Chem. 1967 Nov 10;242(21):5014-8 PMID: 4228672
  7. Antibiotics as tools for metabolic studies. I. A survey of toxic antibiotics in respiratory, phosphorylative and glycolytic systems.
    Arch Biochem Biophys. 1958 Dec;78(2):587-97 PMID: 13618041
  8. ANTIBIOTIC STUDIES. II. INHIBITION OF PHOSPHORYL TRANSFER IN MITOCHONDRIA BY OLIGOMYCIN AND AUROVERTIN.
    Biochemistry. 1964 Dec;3:1961-8 PMID: 14269318
  9. ON THE MECHANISM OF OXIDATIVE PHOSPHORYLATION. VII. THE ENERGY-REQUIRING REDUCTION OF PYRIDINE NUCLEOTIDE BY SUCCINATE AND THE ENERGY-YIELDING OXIDATION OF REDUCED PYRIDINE NUCLEOTIDE BY FUMARATE.
    Biochemistry. 1963 May-Jun;2:523-8 PMID: 14069541
  10. Oxidative phosphorylation in mitochondria.
    Annu Rev Biochem. 1969;38:991-1034 PMID: 4240951
  11. A simplified method for the quantitative assay of small amounts of protein in biologic material.
    Anal Biochem. 1973 Feb;51(2):654-5 PMID: 4735559
  12. The biochemistry of organotin compounds: trialkyltins and oxidative phosphorylation.
    Biochem J. 1958 Jul;69(3):367-76 PMID: 13560377
  13. The structure of mitochondrial ATPase.
    Biochim Biophys Acta. 1973 Dec 31;301(3):249-77 PMID: 4273937
  14. Partial resolution of the enzyme catalyzing oxidative phosphorylation. XXII. Interaction between mitochondrial adenosine triphosphatase inhibitor and mitochondrial adenosine triphosphatase.
    J Biol Chem. 1970 Mar 25;245(6):1336-44 PMID: 4245874
  15. Measurement of molecular weights by electrophoresis on SDS-acrylamide gel.
    Methods Enzymol. 1972;26:3-27 PMID: 4680711
  16. The identification of the site of action of NN'-dicyclohexylcarbodi-imide as a proteolipid in mitochondrial membranes.
    Biochem J. 1971 Nov;125(1):169-77 PMID: 5158902
  17. Assembly of the mitochondrial membrane system. V. Properties of a dispersed preparation of the rutamycin-sensitive adenosine triphosphatase of yeast mitochondria.
    J Biol Chem. 1971 Dec 10;246(23):7328-36 PMID: 4256832
  18. Solubilization of mitochondrial ATPase by phospholipids.
    Biochem Biophys Res Commun. 1972 Jul 25;48(2):341-7 PMID: 4261366
  19. The effect of aurovertin on a soluble mitochondrial adenosine triphosphatase.
    Biochem J. 1967 Sep;104(3):54C-55C PMID: 4227822
  20. ANTIBIOTICS AS TOOLS FOR METABOLIC STUDIES. IV. COMPARATIVE EFFECTIVENESS OF OLIGOMYCINS A, B, C, AND RUTAMYCIN AS INHIBITORS OF PHOSPHORYL TRANSFER REACTIONS IN MITOCHONDRIA.
    Biochemistry. 1965 Mar;4:552-4 PMID: 14311628
  21. Histochemistry of hepatic phosphatases of a physiologic pH; with special reference to the demonstration of bile canaliculi.
    Am J Clin Pathol. 1957 Jan;27(1):13-23 PMID: 13410831
  22. On the subunit structure of soluble mitochondrial ATPase.
    FEBS Lett. 1974 Jul 15;43(2):212-4 PMID: 4277595
  23. Partial resolution of the enzymes catalyzing oxidative phosphorylation. I. Purification and properties of soluble dinitrophenol-stimulated adenosine triphosphatase.
    J Biol Chem. 1960 Nov;235:3322-9 PMID: 13738472
  24. Partial resolution of the enzymes catalyzing oxidative phosphorylation. 8. Properties of a factor conferring oligomycin sensitivity on mitochondrial adenosine triphosphatase.
    J Biol Chem. 1966 May 25;241(10):2461-6 PMID: 4223640
  25. The subunit composition of the mitochondrial oligomycin-insensitive ATPase.
    FEBS Lett. 1971 Oct 1;17(2):327-329 PMID: 11946060
  26. Dicyclohexylcarbodiimide--an inhibitor of oxidative phosphorylation.
    Biochem Biophys Res Commun. 1966 Apr 6;23(1):75-80 PMID: 5928897
  27. Adenosine triphosphatase from rat liver mitochondria. I. Purification, homogeneity, and physical properties.
    J Biol Chem. 1971 Aug 25;246(16):4987-94 PMID: 4255092
  28. STUDIES ON OXIDATIVE PHOSPHORYLATION. X. A COUPLING ENZYME WHICH ACTIVATES REVERSED ELECTRON TRANSFER.
    J Biol Chem. 1965 Jun;240:2644-53 PMID: 14304880
  29. Studies on the mitochondrial oligomycin-insensitivt ATPase. I. An improved method of purification and the behavior of the enzyme in solutions of various depolymerizing agents.
    Arch Biochem Biophys. 1970 Sep;140(1):257-66 PMID: 4248271
  30. A NATURALLY OCCURRING INHIBITOR OF MITOCHONDRIAL ADENOSINE TRIPHOSPHATASE.
    J Biol Chem. 1963 Nov;238:3762-9 PMID: 14109217
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1480533
Published
1975-06-00
Pages
533-7
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1165573
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1480533 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2