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PMID: 3840588 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of a gene from chromosome 1D of wheat encoding a HMW-glutenin subunit.

Nucleic acids research ·Vol. 13 ·No. 19 ·1985-10-11 ·Pages 6833-46

Thompson RD, Bartels D, Harberd NP

Abstract

A high molecular weight glutenin gene in hexaploid wheat has been isolated by cloning in bacteriophage lambda and characterized. The gene corresponds to polypeptide 12 encoded by chromosome 1D in the variety "Chinese Spring". The coding sequence predicted contains seven cysteine residues six of which flank a central repetitive region comprising more than 70% of the polypeptide. These findings are related to the role of high molecular weight subunits in the viscoelastic theory of gluten structure.

MeSH Terms
Amino Acid Sequence Base Sequence Chromosome Mapping Cloning, Molecular Genes Glutens/analogs & derivatives,genetics Macromolecular Substances Molecular Weight Repetitive Sequences, Nucleic Acid Triticum/genetics
Chemicals
Macromolecular Substances Glutens glutenin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Thompson R D
Bartels D
Harberd N P
References (21)
21 references, click to expand
  1. Lambda replacement vectors carrying polylinker sequences.
    J Mol Biol. 1983 Nov 15;170(4):827-42 PMID: 6315951
  2. Random subcloning of sonicated DNA: application to shotgun DNA sequence analysis.
    Anal Biochem. 1983 Feb 15;129(1):216-23 PMID: 6305233
  3. Use of a recombination-deficient phage lambda system to construct wheat genomic libraries.
    Gene. 1984 Oct;30(1-3):237-40 PMID: 6096216
  4. Multiple levels of gene control in eukaryotic cells.
    Nature. 1984 Nov 22-28;312(5992):308-9 PMID: 6504145
  5. Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing.
    Nucleic Acids Res. 1982 Aug 11;10(15):4731-51 PMID: 7133997
  6. In Vitro Synthesis of Wheat (Triticum aestivum L.) Storage Proteins.
    Plant Physiol. 1981 Sep;68(3):778-83 PMID: 16661998
  7. "Nonrandom" DNA sequence analysis in bacteriophage M13 by the dideoxy chain-termination method.
    Proc Natl Acad Sci U S A. 1982 Jul;79(14):4298-302 PMID: 6956859
  8. Lambda Charon vectors (Ch32, 33, 34 and 35) adapted for DNA cloning in recombination-deficient hosts.
    Gene. 1983 Dec;26(2-3):171-9 PMID: 6323258
  9. Nucleic acid sequence and chromosome assignment of a wheat storage protein gene.
    Nucleic Acids Res. 1984 Nov 12;12(21):8129-44 PMID: 6095191
  10. The characterization of cDNA clones coding for wheat storage proteins.
    Nucleic Acids Res. 1983 May 25;11(10):2961-77 PMID: 6190127
  11. Molecular evolution of the seed storage proteins of barley, rye and wheat.
    J Mol Biol. 1985 Jun 5;183(3):499-502 PMID: 4020867
  12. A 12-base-pair DNA motif that is repeated several times in metallothionein gene promoters confers metal regulation to a heterologous gene.
    Proc Natl Acad Sci U S A. 1984 Dec;81(23):7318-22 PMID: 6095286
  13. Delayed onset of regular respiration and subsequent development.
    Early Hum Dev. 1984 Apr;9(3):225-39 PMID: 6734485
  14. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  15. An interactive graphics program for comparing and aligning nucleic acid and amino acid sequences.
    Nucleic Acids Res. 1982 May 11;10(9):2951-61 PMID: 7099970
  16. Empirical predictions of protein conformation.
    Annu Rev Biochem. 1978;47:251-76 PMID: 354496
  17. Pattern recognition in nucleic acid sequences. I. A general method for finding local homologies and symmetries.
    Nucleic Acids Res. 1982 Jan 11;10(1):247-63 PMID: 6801626
  18. Conservation and variability of wheat alpha/beta-gliadin genes.
    Nucleic Acids Res. 1985 Jun 11;13(11):3905-916 PMID: 3839304
  19. Secretion and membrane localization of proteins in Escherichia coli.
    CRC Crit Rev Biochem. 1980;7(4):339-71 PMID: 6993100
  20. Very short repeats and coordinate induction of genes.
    Nature. 1983 Feb 10;301(5900):468-70 PMID: 6823326
  21. Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
    J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
Published
1985-10-11
Pages
6833-46
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322008
Subset
IM
Databases
GENBANK
X03041
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