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PMID: 6323258 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Lambda Charon vectors (Ch32, 33, 34 and 35) adapted for DNA cloning in recombination-deficient hosts.

Gene ·Vol. 26 ·No. 2-3 ·1983-12-00 ·Pages 171-9

Loenen WA, Blattner FR

Abstract

New phage lambda-based cloning vectors, Charons 32, 33, 34 and 35, have been constructed. These vectors allow cloning of large (19-21 kb) DNA fragments in up to six cloning sites. DNA cloned in these vectors can be propagated on recA- Escherichia coli hosts.

MeSH Terms
Bacteriophage lambda/genetics Cloning, Molecular DNA Restriction Enzymes DNA, Recombinant Escherichia coli/genetics Genetic Vectors Recombination, Genetic
Chemicals
DNA, Recombinant DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Loenen W A
Blattner F R
Article Info
Journal
Gene
Abbr.
Gene
ISSN
0378-1119
DOI
10.1016/0378-1119(83)90187-7
Published
1983-12-00
Pages
171-9
Language
English
Region
Netherlands
NLM ID
7706761
Subset
IM
External Links
PubMed source DOI: 10.1016/0378-1119(83)90187-7 View journal details
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