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PMID: 3479796 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cell-cycle-specific interaction of nuclear DNA-binding proteins with a CCAAT sequence from the human thymidine kinase gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 23 ·1987-12-00 ·Pages 8350-4

Knight GB, Gudas JM, Pardee AB

Abstract

Induction of thymidine kinase parallels the onset of DNA synthesis. To investigate the transcriptional regulation of the thymidine kinase gene, we have examined whether specific nuclear factors interact in a cell-cycle-dependent manner with sequences upstream of this gene. Two inverted CCAAT boxes near the transcriptional initiation sites were observed to form complexes with nuclear DNA-binding proteins. The nature of the complexes changes dramatically as the cells approach DNA synthesis and correlates well with the previously reported transcriptional increase of the thymidine kinase gene.

MeSH Terms
Binding Sites Binding, Competitive Cell Cycle DNA Replication DNA-Binding Proteins/metabolism Humans Nuclear Proteins/metabolism Oligonucleotides/metabolism Promoter Regions, Genetic Protein Binding RNA, Messenger/genetics Regulatory Sequences, Nucleic Acid Thymidine Kinase/genetics Transcription, Genetic
Chemicals
DNA-Binding Proteins Nuclear Proteins Oligonucleotides RNA, Messenger Thymidine Kinase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Knight G B
Department of Pharmacology, Harvard Medical School, Boston, MA.
Gudas J M
Pardee A B
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.23.8350
Published
1987-12-00
Pages
8350-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC299540
Subset
IM
Grants
NIGMS NIH HHS · GM 24571 · United States
NCI NIH HHS · T32 CA09361 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.23.8350 PMC Full Text View journal details
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