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PMID: 3473491 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Proximal and distal regulatory elements that influence in vivo expression of a cell cycle-dependent human H4 histone gene.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 12 ·1987-06-00 ·Pages 3982-6

Kroeger P, Stewart C, Schaap T, van Wijnen A, Hirshman J, Helms S, Stein G, Stein J

Abstract

We have examined the sequences required in vivo to promote transcription of a cell cycle-regulated human H4 histone gene. Deletion mutants of the 5' flanking region were assayed in mouse cells or fused with the chloramphenicol acetyltransferase (CAT) gene for assay in HeLa cells. The functional limits of the regulatory sequences were shown to extend at least 6.5 kilobases (kb) upstream. Sequences sufficient for correctly initiated transcription were found in the 70 base pairs (bp) immediately 5' to the cap site. A proximal element located 200-400 bp upstream increased the level of transcription several times above the basal level, although not to maximal levels. Maximal levels of expression were achieved with 6.5 kb of 5' flanking sequence adjacent to the proximal promoter sequences or when a distal enhancer element with both position- and orientation-independent function was moved proximal to the promoter. Our results indicate that a series of 5' cis-acting sequences are functionally related to the fidelity and level of expression of this human H4 histone gene.

MeSH Terms
Animals Cell Cycle Cell Line Chromosome Deletion Cloning, Molecular Genes Genes, Regulator HeLa Cells/cytology,metabolism Histones/genetics Humans L Cells/metabolism Mice Mutation Plasmids RNA, Messenger/genetics,isolation & purification Thymidine Kinase/deficiency,genetics Transcription, Genetic
Chemicals
Histones RNA, Messenger Thymidine Kinase
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Kroeger P
Stewart C
Schaap T
van Wijnen A
Hirshman J
Helms S
Stein G
Stein J
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.12.3982
Published
1987-06-00
Pages
3982-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC305005
Subset
IM
Grants
NIGMS NIH HHS · GM 32010 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.12.3982 PMC Full Text View journal details
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