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PMID: 3029724 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

A nuclear protein with affinity for the 5' flanking region of a cell cycle dependent human H4 histone gene in vitro.

Nucleic acids research ·Vol. 15 ·No. 4 ·1987-02-25 ·Pages 1679-98

van Wijnen AJ, Stein JL, Stein GS

Abstract

A nuclear protein with affinity for the 5' flanking region of a cell cycle dependent human H4 histone gene has been partially purified from nuclear extracts of human HeLa S3 cells. The region involved in the binding of the protein has been localized to an upstream DNA segment using an electrophoretic mobility shift assay. This DNA segment is devoid of RNA polymerase II consensus sequences and contains both homopurinic and A/T rich tracts. Analogous experiments have identified a similar, and perhaps identical, factor that has affinity for a cell cycle dependent human H3 histone gene promoter. This protein appears to bind to a DNA segment containing A/T rich sequences that bear homology with the binding region of the H4 histone promoter. Cell synchronization experiments have shown that the overall affinity of the protein(s) for the H3 and H4 histone 5' flanking regions in vitro is not dramatically altered during the cell cycle. Although the rate of histone gene transcription is modulated during early S phase, transcription occurs throughout the cell cycle. Hence, the protein(s) we have detected here may play a role in the basal expression of these genes.

MeSH Terms
Cell Cycle Cell Nucleus/metabolism DNA Restriction Enzymes DNA, Neoplasm/metabolism Genes Genes, Regulator HeLa Cells/metabolism Histones/genetics Humans Nucleoproteins/metabolism Protein Binding
Chemicals
DNA, Neoplasm Histones Nucleoproteins DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
van Wijnen A J
Stein J L
Stein G S
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33 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/15.4.1679
Published
1987-02-25
Pages
1679-98
Language
English
Region
England
NLM ID
0411011
PMCID
PMC340574
Subset
IM
Grants
NIGMS NIH HHS · GM32010 · United States
External Links
PubMed source DOI: 10.1093/nar/15.4.1679 PMC Full Text View journal details
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