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PMID: 3458197 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Cell cycle-dependent expression of a stable episomal human histone gene in a mouse cell.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 83 ·No. 8 ·1986-04-00 ·Pages 2315-9

Green L, Schlaffer I, Wright K, Moreno ML, Berand D, Hager G, Stein J, Stein G

Abstract

We have constructed a recombinant plasmid that includes a cell cycle-dependent human H4 histone gene with 650 base pairs of 5' and 900 base pairs of 3' flanking sequences and the 69% transforming fragment of bovine papilloma virus. When transfected into C127 mouse cells, this plasmid is maintained as a stable episome with approximately 20 copies per cell. Micrococcal nuclease digestion indicates that the episomal human histone gene is packaged as chromatin. The human H4 histone transcript is initiated at the correct 5' start site and undergoes selective destabilization when DNA synthesis is inhibited. When C127 cells containing the episomal H4 histone gene are synchronized, the human H4 histone mRNA levels are regulated coordinately with DNA replication and parallel those of transcripts from the murine chromosomal H4 histone genes. Our results suggest that the regulatory sequences and/or regulatory molecules associated with murine and human histone genes are compatible. The human histone gene-bovine papillomavirus episome is therefore a viable system for studying cell cycle-regulated histone gene expression under conditions where control is not influenced at the site of chromosomal integration by cis-acting elements of genes normally not contiguous.

MeSH Terms
Animals Cell Cycle Cell Line DNA Replication Extrachromosomal Inheritance Gene Expression Regulation Genes Genes, Regulator Histones/genetics Humans Mice Replicon
Chemicals
Histones
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Green L
Schlaffer I
Wright K
Moreno M L
Berand D
Hager G
Stein J
Stein G
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41 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.83.8.2315
Published
1986-04-00
Pages
2315-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC323287
Subset
IM
Grants
NIGMS NIH HHS · GM32010 · United States
External Links
PubMed source DOI: 10.1073/pnas.83.8.2315 PMC Full Text View journal details
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