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PMID: 3974574 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Identification of promoter elements necessary for transcriptional regulation of a human histone H4 gene in vitro.

Molecular and cellular biology ·Vol. 5 ·No. 2 ·1985-02-00 ·Pages 380-9

Hanly SM, Bleecker GC, Heintz N

Abstract

We have examined the nucleotide sequences necessary for transcription of a human histone H4 gene in vitro. Maximal transcription of the H4 promoter requires, in addition to the TATA box and cap site, promoter elements between 70 and 110 nucleotides upstream from the transcription initiation site. These distal promoter elements are recognized preferentially in extracts from synchronized S-phase HeLa cells. The inability of non-S-phase nuclear extracts to recognize the H4 upstream sequences reflects a specific lack of a transcription factor which interacts with those sequences. These results indicate that the cell cycle regulation of human histone gene expression involves both a specific transcription factor and distal transcription signals in the H4 promoter.

MeSH Terms
Base Sequence Chromosome Deletion Genetic Complementation Test Histones/genetics Humans Mutation Promoter Regions, Genetic Transcription, Genetic
Chemicals
Histones
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hanly S M
Bleecker G C
Heintz N
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22 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.2.380-389.1985
Published
1985-02-00
Pages
380-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366721
Subset
IM
Grants
NIGMS NIH HHS · GM 32544 · United States
Databases
GENBANK
M10231
External Links
PubMed source DOI: 10.1128/mcb.5.2.380-389.1985 PMC Full Text View journal details
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