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PMID: 2864691 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A unique subspecies of histone H4 mRNA from rat myoblasts contains poly(A).

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 20 ·1985-10-00 ·Pages 6760-4

Bird RC, Jacobs FA, Stein G, Stein J, Sells BH

Abstract

Fractionation of rat L6 myoblast histone H4 mRNA into its three component subspecies revealed that one of the major subspecies (H4-1) contained poly(A). The unique poly(A)+ H4 mRNA makes up about 8% of the total polysomal H4 mRNA population detected. Unlike the poly(A)- histone mRNAs, whose levels are reduced by greater than 95% when myoblasts differentiate into myotubes, the poly(A)+ subspecies is reduced by only 70%. The poly(A)+ H4 mRNA from myotubes incubated with actinomycin D decays with a half-life of 37-42 min, which is similar to that obtained for the poly(A)- H4 mRNAs in myoblasts. Both the poly(A)+ and poly(A)- subspecies decay at an increased rate after inhibition of DNA synthesis. In myoblasts the poly(A)+ H4 mRNA exists almost exclusively in the polysomal compartment (greater than 95%) with little (less than 5%) in the free ribonucleoprotein (mRNA-protein or mRNP) complex compartment of the cell. Poly(A)- histone H4 mRNA subspecies, on the other hand, are distributed with approximately 80% in the polysomal compartment and 20% in the free mRNP complex compartment. The unique poly(A)+ H4 mRNA is unusual, not only in that it contains poly(A) but also in its behavior compared to poly(A)- H4 mRNAs during terminal differentiation.

MeSH Terms
Animals Base Sequence Cell Line Histones/genetics Kinetics Molecular Weight Muscles/metabolism Poly A/genetics,isolation & purification RNA, Messenger/genetics,isolation & purification Rats
Chemicals
Histones RNA, Messenger Poly A
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bird R C
Jacobs F A
Stein G
Stein J
Sells B H
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.20.6760
Published
1985-10-00
Pages
6760-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390766
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.20.6760 PMC Full Text View journal details
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