Home LiteratureArticle Details
PMID: 7008028 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Yeast histone mRNA is polyadenylated.

Nucleic acids research ·Vol. 8 ·No. 23 ·1980-12-11 ·Pages 5725-37

Fahrner K, Yarger J, Hereford L

Abstract

Histone mRNA from S. cerevisiae has been identified and partially purified. The RNA is quantitatively retained on oligo (dT) cellulose or poly(U) sepharose as assayed by in vitro translation or hybridization of radiolabelled cloned yeast histone sequences to RNA immobilized on DBM paper. Retention of yeast histone mRNA on either of these chromatographic systems is most likely the result of polyadenylation since, when primed with oligo (dT), the RNA is an extremely good template for reverse transcriptase, as determined by hybrid arrest translation or by hybridization to D. melanogaster histone DNA sequences.

MeSH Terms
Base Sequence Chromatography, Affinity Cloning, Molecular Histones/genetics Nucleic Acid Hybridization Poly A/genetics Protein Biosynthesis RNA, Messenger/genetics,isolation & purification Saccharomyces cerevisiae/genetics
Chemicals
Histones RNA, Messenger Poly A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fahrner K
Yarger J
Hereford L
References (23)
23 references, click to expand
  1. Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1159-66 PMID: 4894690
  2. Polyadenylic acid-containing RNA in Xenopus laevis oocytes.
    J Mol Biol. 1974 May 5;85(1):87-101 PMID: 4835729
  3. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.
    Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330-4 PMID: 4525168
  4. A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels.
    Eur J Biochem. 1974 Jul 1;46(1):83-8 PMID: 4850204
  5. Maternal messenger RNA and histone synthesis in embryos of the surf clam Spisula solidissima.
    Dev Biol. 1975 Apr;43(2):254-63 PMID: 1168601
  6. Nucleotide sequence of the rightward operator of phage lambda.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):1184-8 PMID: 1055375
  7. Chromosomal proteins and chromatin structure.
    Annu Rev Biochem. 1975;44:725-74 PMID: 1094921
  8. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  9. Identification and fractionation of plant histones.
    Arch Biochem Biophys. 1976 Oct;176(2):510-8 PMID: 984846
  10. Studies of human histone messenger RNA. II. The resolution of fractions containing individual human histone messenger RNA species.
    J Biol Chem. 1977 Jan 10;252(1):173-80 PMID: 833117
  11. Number and distribution of polyadenylated RNA sequences in yeast.
    Cell. 1977 Mar;10(3):453-62 PMID: 321129
  12. Regulation of a set of abundant mRNA sequences.
    Cell. 1977 Mar;10(3):463-7 PMID: 321130
  13. Differential accumulation of two size classes of poly(A) associated with messenger RNA during oogenesis in Xenopus laevis.
    Dev Biol. 1977 Jun;57(2):427-39 PMID: 873054
  14. Cell-free translation analysis of messenger RNA in echinoderm and amphibian early development.
    Dev Biol. 1977 Oct 1;60(1):48-68 PMID: 902930
  15. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350-4 PMID: 414220
  16. A portion of all major classes of histone messenger RNA in amphibian oocytes is polyadenylated.
    J Biol Chem. 1978 Mar 25;253(6):2018-25 PMID: 564902
  17. The organization of the histone genes in Drosophila melanogaster: functional and evolutionary implications.
    Cold Spring Harb Symp Quant Biol. 1978;42 Pt 2:1047-51 PMID: 98262
  18. Yeast inner histones and the evolutionary conservation of histone-histone interactions.
    Biochemistry. 1978 Sep 5;17(18):3825-33 PMID: 359045
  19. Rate of macromolecular synthesis through the cell cycle of the yeast Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1978 Sep;75(9):4384-8 PMID: 360219
  20. Large-scale preparative polyacrylamide gel electrophoresis of ribonucleic acid.
    Anal Biochem. 1979 Mar;93(2):299-305 PMID: 380392
  21. Modulations of histone messenger RNA during the early development of Xenopus laevis.
    Dev Biol. 1979 Jul;71(1):71-82 PMID: 572787
  22. Histone genes and histone messengers.
    Annu Rev Biochem. 1979;48:837-70 PMID: 112914
  23. Isolation of yeast histone genes H2A and H2B.
    Cell. 1979 Dec;18(4):1261-71 PMID: 519767
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/8.23.5725
Published
1980-12-11
Pages
5725-37
Language
English
Region
England
NLM ID
0411011
PMCID
PMC324337
Subset
IM
Grants
NIGMS NIH HHS · GM 23549 · United States
NIGMS NIH HHS · GM27334 · United States
External Links
PubMed source DOI: 10.1093/nar/8.23.5725 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2