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PMID: 3413063 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequences contained within the promoter of the human thymidine kinase gene can direct cell-cycle regulation of heterologous fusion genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 16 ·1988-08-00 ·Pages 5894-8

Kim YK, Wells S, Lau YF, Lee AS

Abstract

Recent evidence on the transcriptional regulation of the human thymidine kinase (TK) gene raises the possibility that cell-cycle regulatory sequences may be localized within its promoter. A hybrid gene that combines the TK 5' flanking sequence and the coding region of the bacterial neomycin-resistance gene (neo) has been constructed. Upon transfection into a hamster fibroblast cell line K12, the hybrid gene exhibits cell-cycle-dependent expression. Deletion analysis reveals that the region important for cell-cycle regulation is within -441 to -63 nucleotides from the transcriptional initiation site. This region (-441 to -63) also confers cell-cycle regulation to the herpes simplex virus thymidine kinase (HSVtk) promoter, which is not expressed in a cell-cycle manner. We conclude that the -441 to -63 sequence within the human TK promoter is important for cell-cycle-dependent expression.

MeSH Terms
Base Sequence Cell Cycle Cloning, Molecular Humans Promoter Regions, Genetic RNA, Messenger/analysis Thymidine Kinase/genetics Transcription, Genetic
Chemicals
RNA, Messenger Thymidine Kinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Kim Y K
Department of Biochemistry, University of Southern California School of Medicine, Los Angeles 90033.
Wells S
Lau Y F
Lee A S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.16.5894
Published
1988-08-00
Pages
5894-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC281871
Subset
IM
Grants
NIGMS NIH HHS · GM31138 · United States
External Links
PubMed source DOI: 10.1073/pnas.85.16.5894 PMC Full Text View journal details
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