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PMID: 2991860 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human N-ras: cDNA cloning and gene structure.

Nucleic acids research ·Vol. 13 ·No. 14 ·1985-07-25 ·Pages 5255-68

Hall A, Brown R

Abstract

The structure and organisation of the human N-ras gene has been determined by analysing cDNA clones derived from the two main mRNA transcripts. One clone in particular is 4.1 Kb long and originates from the larger (4.3 Kb) message. Sequence analysis of this clone has revealed that the N-ras gene consists of seven exons. A second clone deriving from the smaller (2 Kb) message shows that the difference between the two transcripts is a simple extension through the termination site of the 2 Kb transcript. Using S1 analysis, two transcriptional starts have been mapped, 10 bp apart. There is no obvious TATA box in the expected promoter region of the gene, though there are 4 GGGCGG sequences surrounding the start sites. The 5' untranslated sequence contains 2 ATGs upstream of the initiation codon.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/analysis DNA Restriction Enzymes/metabolism Endonucleases/metabolism Humans Oncogenes Single-Strand Specific DNA and RNA Endonucleases
Chemicals
DNA Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Hall A
Brown R
References (41)
41 references, click to expand
  1. The HL-60 transforming sequence: a ras oncogene coexisting with altered myc genes in hematopoietic tumors.
    Cell. 1983 Jul;33(3):749-57 PMID: 6683594
  2. Identification of transforming gene in two human sarcoma cell lines as a new member of the ras gene family located on chromosome 1.
    Nature. 1983 Jun 2-8;303(5916):396-400 PMID: 6304521
  3. Structure and organization of the human Ki-ras proto-oncogene and a related processed pseudogene.
    Nature. 1983 Aug 11-17;304(5926):501-6 PMID: 6308466
  4. Activation of Ki-ras2 gene in human colon and lung carcinomas by two different point mutations.
    Nature. 1983 Aug 11-17;304(5926):507-13 PMID: 6308467
  5. Transforming genes of human hematopoietic tumors: frequent detection of ras-related oncogenes whose activation appears to be independent of tumor phenotype.
    Proc Natl Acad Sci U S A. 1983 Aug;80(16):4926-30 PMID: 6308661
  6. Structure and activation of the human N-ras gene.
    Cell. 1983 Sep;34(2):581-6 PMID: 6616621
  7. The promoter-specific transcription factor Sp1 binds to upstream sequences in the SV40 early promoter.
    Cell. 1983 Nov;35(1):79-87 PMID: 6313230
  8. A simple and very efficient method for generating cDNA libraries.
    Gene. 1983 Nov;25(2-3):263-9 PMID: 6198242
  9. Activation of N-ras gene in bone marrow cells from a patient with acute myeloblastic leukaemia.
    Nature. 1984 Feb 2-8;307(5950):476-8 PMID: 6582353
  10. Compilation and analysis of sequences upstream from the translational start site in eukaryotic mRNAs.
    Nucleic Acids Res. 1984 Jan 25;12(2):857-72 PMID: 6694911
  11. The human c-Ha-ras2 is a processed pseudogene inactivated by numerous base substitutions.
    Nucleic Acids Res. 1984 Feb 24;12(4):1821-8 PMID: 6322122
  12. Structure, expression, and mutation of the hypoxanthine phosphoribosyltransferase gene.
    Proc Natl Acad Sci U S A. 1984 Apr;81(7):2147-51 PMID: 6326107
  13. Analysis of the transforming potential of the human H-ras gene by random mutagenesis.
    Proc Natl Acad Sci U S A. 1984 Jul;81(13):4008-12 PMID: 6330729
  14. Mechanism of activation of an N-ras gene in the human fibrosarcoma cell line HT1080.
    EMBO J. 1984 Jun;3(6):1321-6 PMID: 6086315
  15. HMG CoA reductase: a negatively regulated gene with unusual promoter and 5' untranslated regions.
    Cell. 1984 Aug;38(1):275-85 PMID: 6088070
  16. Comparative biochemical properties of normal and activated human ras p21 protein.
    Nature. 1984 Aug 23-29;310(5979):644-9 PMID: 6147754
  17. Intrinsic GTPase activity distinguishes normal and oncogenic ras p21 molecules.
    Proc Natl Acad Sci U S A. 1984 Sep;81(18):5704-8 PMID: 6148751
  18. Homologies between signal transducing G proteins and ras gene products.
    Science. 1984 Nov 16;226(4676):860-2 PMID: 6436980
  19. Effect of the 21-bp repeat upstream element on in vitro transcription from the early and late SV40 promoters.
    EMBO J. 1984 Oct;3(10):2373-82 PMID: 6094181
  20. Transcription of the mouse dihydrofolate reductase gene proceeds unabated through seven polyadenylation sites and terminates near a region of repeated DNA.
    Mol Cell Biol. 1984 Dec;4(12):2921-4 PMID: 6084812
  21. Adenosine deaminase: characterization and expression of a gene with a remarkable promoter.
    EMBO J. 1985 Feb;4(2):437-43 PMID: 3839456
  22. 3' non-coding region sequences in eukaryotic messenger RNA.
    Nature. 1976 Sep 16;263(5574):211-4 PMID: 822353
  23. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  24. Transforming genes of carcinomas and neuroblastomas introduced into mouse fibroblasts.
    Nature. 1981 Mar 19;290(5803):261-4 PMID: 7207618
  25. Speculations on RNA splicing.
    Cell. 1981 Mar;23(3):643-6 PMID: 7226224
  26. Transforming activity of human tumor DNAs.
    Proc Natl Acad Sci U S A. 1981 Feb;78(2):1181-4 PMID: 6940134
  27. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  28. Isolation and preliminary characterization of a human transforming gene from T24 bladder carcinoma cells.
    Nature. 1982 Apr 1;296(5856):404-9 PMID: 7063039
  29. Human EJ bladder carcinoma oncogene is homologue of Harvey sarcoma virus ras gene.
    Nature. 1982 Jun 10;297(5866):474-8 PMID: 6283357
  30. Tumorigenic transformation of mammalian cells induced by a normal human gene homologous to the oncogene of Harvey murine sarcoma virus.
    Nature. 1982 Jun 10;297(5866):479-83 PMID: 6283358
  31. T24 human bladder carcinoma oncogene is an activated form of the normal human homologue of BALB- and Harvey-MSV transforming genes.
    Nature. 1982 Jul 22;298(5872):343-7 PMID: 6283384
  32. Oncogenes in human tumor cell lines: molecular cloning of a transforming gene from human bladder carcinoma cells.
    Proc Natl Acad Sci U S A. 1982 May;79(9):2845-9 PMID: 6953433
  33. Transforming genes of human bladder and lung carcinoma cell lines are homologous to the ras genes of Harvey and Kirsten sarcoma viruses.
    Proc Natl Acad Sci U S A. 1982 Jun;79(11):3637-40 PMID: 6285355
  34. High-efficiency cloning of full-length cDNA.
    Mol Cell Biol. 1982 Feb;2(2):161-70 PMID: 6287227
  35. Human genome contains four genes homologous to transforming genes of Harvey and Kirsten murine sarcoma viruses.
    Proc Natl Acad Sci U S A. 1982 Aug;79(16):4848-52 PMID: 6289320
  36. Mechanism of activation of a human oncogene.
    Nature. 1982 Nov 11;300(5888):143-9 PMID: 6290897
  37. A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene.
    Nature. 1982 Nov 11;300(5888):149-52 PMID: 7133135
  38. Activation of the T24 bladder carcinoma transforming gene is linked to a single amino acid change.
    Nature. 1982 Dec 23;300(5894):762-5 PMID: 7177195
  39. Altered gene products are associated with activation of cellular rasK genes in human lung and colon carcinomas.
    Cell. 1983 Jan;32(1):201-8 PMID: 6825168
  40. Complete nucleotide sequences of the T24 human bladder carcinoma oncogene and its normal homologue.
    Nature. 1983 Mar 3;302(5903):33-7 PMID: 6298635
  41. Sequence and structure of the coding region of the human H-ras-1 gene from T24 bladder carcinoma cells.
    J Mol Appl Genet. 1983;2(2):173-80 PMID: 6308118
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.14.5255
Published
1985-07-25
Pages
5255-68
Language
English
Region
England
NLM ID
0411011
PMCID
PMC321863
Subset
IM
Databases
GENBANK
X02751
External Links
PubMed source DOI: 10.1093/nar/13.14.5255 PMC Full Text View journal details
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