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PMID: 2849761 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Evolution of the herpes thymidine kinase: identification and comparison of the equine herpesvirus 1 thymidine kinase gene reveals similarity to a cell-encoded thymidylate kinase.

Nucleic acids research ·Vol. 16 ·No. 23 ·1988-12-09 ·Pages 11303-17

Robertson GR, Whalley JM

Abstract

We have identified the equine herpesvirus 1 (EHV-1) thymidine kinase gene (TK) by DNA-mediated transformation and by DNA sequencing. Alignment of the amino acid sequence of the EHV-1 TK with the TKs from 3 other herpesviruses revealed regions of homology, some of which correspond to the previously identified substrate binding sites, while others have as yet, no assigned function. In particular, the strict conservation of an aspartate within the proposed nucleoside binding site suggests a role in ATP binding for this residue. Comparison of 5 herpes TKs with the thymidylate kinase of yeast revealed significant similarity which was strongest in those regions important to catalytic activity of the herpes TKs, and, therefore we propose that the herpes TK may be derived from a cellular thymidylate kinase. The implications for the evolution of enzyme activities within a pathway of nucleotide metabolism are discussed.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cell Line, Transformed Genes Herpesviridae/enzymology Herpesvirus 1, Equid/enzymology,genetics Mice Molecular Sequence Data Nucleoside-Phosphate Kinase/genetics Phosphotransferases/genetics Saccharomyces cerevisiae/enzymology Sequence Homology, Nucleic Acid Thymidine Kinase/genetics,isolation & purification
Chemicals
Phosphotransferases Thymidine Kinase Nucleoside-Phosphate Kinase dTMP kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Robertson G R
School of Biological Sciences, Macquarie University, Sydney, NSW, Australia.
Whalley J M
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/16.23.11303
Published
1988-12-09
Pages
11303-17
Language
English
Region
England
NLM ID
0411011
PMCID
PMC339011
Subset
IM
Databases
GENBANK
X13209
External Links
PubMed source DOI: 10.1093/nar/16.23.11303 PMC Full Text View journal details
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