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PMID: 3019675 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of an Epstein-Barr virus-coded thymidine kinase.

The EMBO journal ·Vol. 5 ·No. 8 ·1986-08-00 ·Pages 1959-66

Littler E, Zeuthen J, McBride AA, Trøst Sørensen E, Powell KL, Walsh-Arrand JE, Arrand JR

Abstract

We have demonstrated the presence of an Epstein-Barr virus (EBV)-coded thymidine kinase (TK) by producing biochemically transformed, TK-positive mammalian cell lines using either microinjection of whole EBV virions or calcium phosphate-mediated transfection of the SalI-B restriction endonuclease fragment of EBV DNA. Analysis of these cell lines showed that: (i) EBV DNA was present in the cell lines, (ii) sequences from the SalI-B restriction endonuclease fragment of EBV were expressed, (iii) a TK activity was present and (iv) a protein with antigenic cross-reactivity with the herpes simplex virus (HSV) TK was produced. The identity of the EBV TK gene was determined by demonstrating that a recombinant plasmid, which expressed the protein product of the BXLF1 open reading frame as a fusion protein, could complement TK- strains of E. coli. A comparison of the predicted amino acid sequences of the TK proteins of EBV and HSV-1 revealed significant regions of homology.

MeSH Terms
Amino Acid Sequence B-Lymphocytes Cell Line Cell Transformation, Viral DNA Restriction Enzymes DNA, Viral/genetics Escherichia coli/genetics Genes Genes, Viral Herpesvirus 4, Human/enzymology,genetics Humans Molecular Weight Nucleic Acid Hybridization Plasmids Thymidine Kinase/genetics Transfection
Chemicals
DNA, Viral Thymidine Kinase DNA Restriction Enzymes
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Littler E
Zeuthen J
McBride A A
Trøst Sørensen E
Powell K L
Walsh-Arrand J E
Arrand J R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1986-08-00
Pages
1959-66
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1167064
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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