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PMID: 2995987 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Locating a nucleotide-binding site in the thymidine kinase of vaccinia virus and of herpes simplex virus by scoring triply aligned protein sequences.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 20 ·1985-10-00 ·Pages 6815-9

Gentry GA

Abstract

Computer techniques were used to locate related segments of amino acid sequences in the thymidine kinases of vaccinia virus and of herpes simplex virus type 1 and in porcine adenylate kinase. As determined by a procedure that evaluates triply aligned sequences, the probability that the similarities among the segments described here arose by chance was no greater than 0.001. Because the sequence in porcine adenylate kinase is a nucleotide phosphate-binding site it is concluded that the segments in the vaccinia virus and herpes simplex virus thymidine kinases perform similar functions. The segments are residues 16-23 in porcine adenylate kinase, 11-19 in vaccinia virus thymidine kinase, and 56-64 in herpes simplex virus thymidine kinase.

MeSH Terms
Adenylate Kinase/metabolism Amino Acid Sequence Animals Binding Sites Nucleotides/metabolism Protein Binding Simplexvirus/enzymology Software Swine Thymidine Kinase/metabolism Vaccinia virus/enzymology
Chemicals
Nucleotides Thymidine Kinase Adenylate Kinase
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Gentry G A
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26 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.20.6815
Published
1985-10-00
Pages
6815-9
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390778
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.20.6815 PMC Full Text View journal details
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