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PMID: 6328003 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Effects of deletions on expression of the herpes simplex virus thymidine kinase gene from the intact viral genome: the amino terminus of the enzyme is dispensable for catalytic activity.

Journal of virology ·Vol. 50 ·No. 3 ·1984-06-00 ·Pages 733-8

Halpern ME, Smiley JR

Abstract

We have transferred two deletions affecting the 5' end of the herpes simplex virus thymidine kinase (TK) gene into the intact viral genome. One, extending from -12 to +189, had no effect on TK mRNA synthesis and only a small effect on TK activity, although the first 27 codons of the TK polypeptide were deleted. The other, extending from -85 to +85, severely impaired TK mRNA synthesis. We conclude that the amino terminus of the TK polypeptide is dispensable for catalytic activity, and that expression of TK in viral infections requires some of the same promoter elements used in uninfected cells.

MeSH Terms
Animals Cell Line Chlorocebus aethiops Chromosome Deletion Genes Genes, Viral Kidney Kinetics Simplexvirus/genetics Thymidine Kinase/genetics,metabolism Transcription, Genetic
Chemicals
Thymidine Kinase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Halpern M E
Smiley J R
References (29)
29 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.50.3.733-738.1984
Published
1984-06-00
Pages
733-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC255731
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.50.3.733-738.1984 PMC Full Text View journal details
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