Home LiteratureArticle Details
PMID: 6248879 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Epstein-Barr virus (B95-8) DNA VII: molecular cloning and detailed mapping.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 5 ·1980-05-00 ·Pages 2999-3003

Dambaugh T, Beisel C, Hummel M, King W, Fennewald S, Cheung A, Heller M, Raab-Traub N, Kieff E

Abstract

Two of the Sal I fragments and all of the internal BamHI fragments (with the exception of BamHI c, a 0.6 x 10(6) dalton fragment) of Epstein-Barr virus (EBV) DNA have been cloned in pBR322. The termini and other parts of the DNA (including the EcoRI fragment which contains BamHI c) have been cloned as EcoRI fragments in bacteriophage Charon 4A. The cloned DNAs have been used to derive a complete map of the BamHI fragments of EBV DNA and to align the BamHI, EcoRI, HindIII, and SalI cleavage sites in EBV DNA.

MeSH Terms
Bacteriocin Plasmids Bacteriophage lambda/genetics Chromosome Mapping Cloning, Molecular/methods DNA Restriction Enzymes/metabolism DNA, Viral/genetics Herpesvirus 4, Human/genetics
Chemicals
DNA, Viral DNA Restriction Enzymes
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Dambaugh T
Beisel C
Hummel M
King W
Fennewald S
Cheung A
Heller M
Raab-Traub N
Kieff E
References (31)
31 references, click to expand
  1. Release of infectious Epstein-Barr virus by transformed marmoset leukocytes.
    Proc Natl Acad Sci U S A. 1973 Jan;70(1):190-4 PMID: 4346033
  2. DNA of Epstein-Barr virus. I. Comparative studies of the DNA of Epstein-Barr virus from HR-1 and B95-8 cells: size, structure, and relatedness.
    J Virol. 1975 Mar;15(3):556-9 PMID: 163373
  3. Studies on the cleavage of bacteriophage lambda DNA with EcoRI Restriction endonuclease.
    J Mol Biol. 1975 Jan 25;91(3):315-28 PMID: 1102702
  4. Proteins of Epstein-Barr virus. I. Analysis of the polypeptides of purified enveloped Epstein-Barr virus.
    J Virol. 1976 Mar;17(3):935-49 PMID: 176452
  5. Covalently closed circular duplex DNA of Epstein-Barr virus in a human lymphoid cell line.
    J Mol Biol. 1976 Apr 15;102(3):511-30 PMID: 178878
  6. Epstein-Barr virus-specific RNA. I. Analysis of viral RNA in cellular extracts and in the polyribosomal fraction of permissive and nonpermissive lymphoblastoid cell lines.
    J Virol. 1976 May;18(2):518-25 PMID: 178893
  7. Charon phages: safer derivatives of bacteriophage lambda for DNA cloning.
    Science. 1977 Apr 8;196(4286):161-9 PMID: 847462
  8. Screening lambdagt recombinant clones by hybridization to single plaques in situ.
    Science. 1977 Apr 8;196(4286):180-2 PMID: 322279
  9. Epstein-barr virus-specific RNA. II. Analysis of polyadenylated viral RNA in restringent, abortive, and prooductive infections.
    J Virol. 1977 May;22(2):321-30 PMID: 194056
  10. Circular Epstein-Barr virus genomes of reduced size in a human lymphoid cell line of infectious mononucleosis origin.
    J Virol. 1977 May;22(2):373-80 PMID: 194059
  11. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I.
    J Mol Biol. 1977 Jun 15;113(1):237-51 PMID: 881736
  12. HindII, HindIII, and HpaI restriction fragment maps of bacteriophage lambda DNA.
    Gene. 1977 Sep;2(1):1-31 PMID: 598704
  13. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
    Gene. 1977;2(2):95-113 PMID: 344137
  14. Cleavage of Epstein-Barr virus DNA by restriction endonucleases EcoRI, HindIII and BamI.
    Nucleic Acids Res. 1978 Apr;5(4):1387-402 PMID: 206875
  15. DNA of Epstein-Barr virus. III. Identification of restriction enzyme fragments that contain DNA sequences which differ among strains of Epstein-Barr virus.
    J Virol. 1978 Aug;27(2):388-98 PMID: 211267
  16. pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long.
    Nucleic Acids Res. 1978 Aug;5(8):2721-8 PMID: 358147
  17. Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase.
    Nature. 1978 Oct 19;275(5681):617-24 PMID: 360074
  18. The isolation of structural genes from libraries of eucaryotic DNA.
    Cell. 1978 Oct;15(2):687-701 PMID: 719759
  19. DNA of Epstein-Barr virus. IV. Linkage map of restriction enzyme fragments of the B95-8 and W91 strains of Epstein-Barr Virus.
    J Virol. 1978 Nov;28(2):524-42 PMID: 214576
  20. Cloning human fetal gamma globin and mouse alpha-type globin DNA: preparation and screening of shotgun collections.
    Science. 1978 Dec 22;202(4374):1279-84 PMID: 725603
  21. A simple method for identifying the palindromic sequences recognized by restriction endonucleases: the nucleotide sequence of the AvaII site.
    Gene. 1978 Sep;4(1):1-23 PMID: 215495
  22. Epstein-Barr virus-specific RNA. III. Mapping of DNA encoding viral RNA in restringent infection.
    J Virol. 1979 Jan;29(1):261-74 PMID: 219221
  23. Epstein-Barr virus RNA in Burkitt tumor tissue.
    Cell. 1979 Feb;16(2):313-22 PMID: 222455
  24. DNA of Epstein-Barr virus. V. Direct repeats of the ends of Epstein-Barr virus DNA.
    J Virol. 1979 Jun;30(3):852-62 PMID: 225546
  25. DNA of Epstein-Barr virus. VI. Mapping of the internal tandem reiteration.
    J Virol. 1979 Aug;31(2):315-24 PMID: 225556
  26. A comprehensive molecular map of bacteriophage lambda.
    Gene. 1979 Nov;7(3-4):217-70 PMID: 160360
  27. Organization of repeated regions within the Epstein-Barr virus DNA molecule.
    J Virol. 1980 Jan;33(1):507-21 PMID: 6245247
  28. DNA of Epstein-Barr virus. II. Comparison of the molecular weights of restriction endonuclease fragments of the DNA of Epstein-Barr virus strains and identification of end fragments of the B95-8 strain.
    J Virol. 1977 Aug;23(2):421-9 PMID: 196117
  29. Analysis of chromosomal integration and deletions of yeast plasmids.
    Nucleic Acids Res. 1977;4(5):1429-48 PMID: 331256
  30. A physical map of the DNA regions flanking the rabbit beta-globin gene.
    Cell. 1977 Oct;12(2):429-39 PMID: 912752
  31. In vitro packaging of a lambda Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1.
    Gene. 1977 May;1(3-4):255-80 PMID: 338419
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.5.2999
Published
1980-05-00
Pages
2999-3003
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349534
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.5.2999 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2