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PMID: 206875 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Cleavage of Epstein-Barr virus DNA by restriction endonucleases EcoRI, HindIII and BamI.

Nucleic acids research ·Vol. 5 ·No. 4 ·1978-04-00 ·Pages 1387-402

Rymo L, Forsblom S

Abstract

The cleavage of the DNAs of the B95-8 and P3HR-1 virus strains of Epstein-Barr virus by the restriction endonucleases EcoRI, HindIII and BamI was investigated using a new technique for quantitative evaluation of the fluorescence of ethidium stained DNA fragments separated on agarose gels. The results obtained with B95-8 DNA showed that in addition to the limited repetitions of nucleotide sequences observed in the EcoRI and HindIII cleavage patterns, the molecule contained a BamI fragment with a molecular mass of 2.0 megadaltons which was present in a total of about 11 copies and localized to a limited part of the DNA molecule. The same sequences were also present in the P3HR-1 DNA albeit in a lower molar ratio. P3HR-1 DNA yielded restriction enzyme cleavage patterns suggesting DNA sequence heterogeneity of P3HR-1 virus. No fragment was present in more than about 4 copies per molecule of P3HR-1 DNA. Comparison of the restriction enzyme cleavage patterns of P3HR-1 and B95-8 DNA revealed a high degree of structural homology emphasized by nucleic acid hybridization experiments with EBV complementary RNA synthesized in vitro.

MeSH Terms
Binding Sites DNA Restriction Enzymes/metabolism DNA, Viral/metabolism Deoxyribonucleotides/analysis Herpesvirus 4, Human Spectrometry, Fluorescence
Chemicals
DNA, Viral Deoxyribonucleotides DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rymo L
Forsblom S
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21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/5.4.1387
Published
1978-04-00
Pages
1387-402
Language
English
Region
England
NLM ID
0411011
PMCID
PMC342086
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/5.4.1387 PMC Full Text View journal details
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