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PMID: 3104905 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

GTP-binding domain: three consensus sequence elements with distinct spacing.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 84 ·No. 7 ·1987-04-00 ·Pages 1814-8

Dever TE, Glynias MJ, Merrick WC

Abstract

A sequence comparison of nine functionally different GTP-binding protein families has yielded further information on the general characterization of the conservation and importance of amino acid sequences in the GTP-binding domain, including a consensus sequence composed of three consensus elements GXXXXGK, DXXG, and NKXD with consensus spacings of either 40-80 or approximately equal to 130-170 amino acid residues between the first and second elements and approximately 40-80 amino acid residues between the second and third sequence elements; the sequence NKXW in place of NKXD in the sequence element responsible for base specificity allows the use of ITP as well as GTP; dGTP can be used with essentially the same efficiency as GTP; signal transducing proteins and enzymes have been identified in the nine families; and family conservations allow the identification of the most probable consensus sequence element when more than one is present. Employing these features we have screened the protein sequence data base of the Protein Identification Resource and have identified only known GTP-binding proteins with the exception of protein 2C from foot-and-mouth disease virus as matching the consensus sequence. Based on this finding we predict that foot-and-mouth disease virus protein 2C binds GTP and, by analogy, that protein 2C from several related viruses (polio, rhino, encephalomyocarditis, and cowpea mosaic) will bind a nucleotide as part of its biologic activity.

MeSH Terms
Amino Acid Sequence Animals Binding Sites GTP-Binding Proteins/metabolism Guanine Guanosine Triphosphate/metabolism Humans Probability Structure-Activity Relationship Viral Proteins/metabolism
Chemicals
Viral Proteins Guanine Guanosine Triphosphate GTP-Binding Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dever T E
Glynias M J
Merrick W C
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.84.7.1814
Published
1987-04-00
Pages
1814-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC304531
Subset
IM
Grants
NIADDK NIH HHS · AM21594 · United States
NIGMS NIH HHS · GM26796 · United States
External Links
PubMed source DOI: 10.1073/pnas.84.7.1814 PMC Full Text View journal details
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