Polypeptide chain elongation factor 1 alpha (EF-1 alpha) from yeast: nucleotide sequence of one of the two genes for EF-1 alpha from Saccharomyces cerevisiae.

The EMBO journal ·Vol. 3 ·No. 8 ·1984-08-00 ·Pages 1825-30

Nagata S, Nagashima K, Tsunetsugu-Yokota Y, Fujimura K, Miyazaki M, Kaziro Y

Abstract

Messenger RNA for yeast cytosolic polypeptide chain elongation factor 1 alpha (EF-1 alpha) was partially purified from Saccharomyces cerevisiae. Double-stranded complementary DNA (cDNA) was synthesized and cloned in Escherichia coli with pBR327 as a vector. Recombinant plasmid carrying yEF-1 alpha cDNA was identified by cross-hybridization with the E. coli tufB gene and the yeast mitochondrial EF-Tu gene (tufM) under non-stringent conditions. A yeast gene library was then screened with the EF-1 alpha cDNA and several clones containing the chromosomal gene for EF-1 alpha were isolated. Restriction analysis of DNA fragments of these clones as well as the Southern hybridization of yeast genomic DNA with labelled EF-1 alpha cDNA indicated that there are two EF-1 alpha genes in S. cerevisiae. The nucleotide sequence of one of the two EF-1 alpha genes (designated as EF1 alpha A) was established together with its 5'- and 3'-flanking sequences. The sequence contained 1374 nucleotides coding for a protein of 458 amino acids with a calculated mol. wt. of 50 300. The derived amino acid sequence showed homologies of 31% and 32% with yeast mitochondrial EF-Tu and E. coli EF-Tu, respectively.

MeSH Terms

Amino Acid Sequence Base Sequence Cloning, Molecular DNA, Recombinant Escherichia coli/genetics Genes Genes, Fungal Peptide Elongation Factor 1 Peptide Elongation Factor Tu Peptide Elongation Factors/genetics RNA, Fungal/genetics RNA, Messenger/genetics Saccharomyces cerevisiae/genetics

Chemicals

DNA, Recombinant Peptide Elongation Factor 1 Peptide Elongation Factors RNA, Fungal RNA, Messenger Peptide Elongation Factor Tu

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Nagata S

Nagashima K

Tsunetsugu-Yokota Y

Fujimura K

Miyazaki M

Kaziro Y

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32 references, click to expand

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Article Info

Journal

The EMBO journal

Abbr.

EMBO J

ISSN

0261-4189

Published

1984-08-00

Pages

1825-30

Language

English

Region

England

NLM ID

8208664

PMCID

PMC557603

Subset

Databases

GENBANK

X00779

External Links

PubMed source PMC Full Text View journal details

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Polypeptide chain elongation factor 1 alpha (EF-1 alpha) from yeast: nucleotide sequence of one of the two genes for EF-1 alpha from Saccharomyces cerevisiae.

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