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PMID: 2989813 Published · ppublish English Journal Article

GTPase of bovine rod outer segments: the amino acid sequence of the alpha subunit as derived from the cDNA sequence.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 13 ·1985-07-00 ·Pages 4316-20

Yatsunami K, Khorana HG

Abstract

The sequence of the 350 amino acids in the alpha subunit of GTPase of bovine rod outer segments has been determined. Enriched GTPase mRNA was used to prepare a cDNA library in the expression vector lambda gt11 and several overlapping cDNA clones corresponding to the alpha subunit of the GTPase were identified. The cDNA sequence determined contains 93 nucleotides upstream of the 5' end of the coding region, 1050 nucleotides that specify the amino acid sequence, and 45 nucleotides downstream from the 3' end. The previously described partial amino acid sequences and the sequences at the ADP-ribosylation sites for cholera and pertussis toxins are all confirmed and fitted into the present complete sequence. Homologies are found between the sequence of the alpha subunit and those of other guanine nucleotide-binding proteins, the ras proteins, peptide chain elongation factors EF-Tu and EF-G, and the initiation factor IF2.

MeSH Terms
Amino Acid Sequence Animals Cattle DNA/analysis DNA Restriction Enzymes/metabolism Electrophoresis, Polyacrylamide Gel GTP Phosphohydrolases/analysis,genetics Macromolecular Substances Phosphoric Monoester Hydrolases/analysis Photoreceptor Cells/enzymology RNA, Messenger/analysis Rod Cell Outer Segment/enzymology
Chemicals
Macromolecular Substances RNA, Messenger DNA DNA Restriction Enzymes Phosphoric Monoester Hydrolases GTP Phosphohydrolases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Yatsunami K
Khorana H G
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50 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.13.4316
Published
1985-07-00
Pages
4316-20
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390404
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.82.13.4316 PMC Full Text View journal details
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