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PMID: 6149748 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Partial cDNA sequence of the gamma subunit of transducin.

Biochemical and biophysical research communications ·Vol. 124 ·No. 1 ·1984-10-15 ·Pages 250-5

Van Dop C, Medynski D, Sullivan K, Wu AM, Fung BK, Bourne HR

Abstract

A 151 bp cDNA segment that encodes the amino-terminal portion of the gamma subunit of bovine transducin was isolated from a retinal cDNA library constructed in the expression vector lambda gt11. The base sequence of this cDNA confirms the sequence of the first 39 amino-terminal amino acids reported for the native protein (McConnell et al. (1984) Fed. Proc. 43, 1585). Northern blot analysis indicates that the complete mRNA is approximately 650 bases long and that its expression is limited to the retina.

MeSH Terms
Amino Acid Sequence Animals Bacteriophage lambda/genetics Base Composition Base Sequence Cattle Cloning, Molecular DNA/analysis Genes Macromolecular Substances Membrane Proteins/genetics Nucleic Acid Hybridization Poly A/genetics RNA, Messenger/genetics Retina/metabolism Transducin
Chemicals
Macromolecular Substances Membrane Proteins RNA, Messenger Poly A DNA Transducin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Van Dop C
Medynski D
Sullivan K
Wu A M
Fung B K
Bourne H R
Article Info
Journal
Biochemical and biophysical research communications
Abbr.
Biochem Biophys Res Commun
ISSN
0006-291X
Published
1984-10-15
Pages
250-5
Language
English
Region
United States
NLM ID
0372516
Subset
IM
Grants
NIGMS NIH HHS · GM-27800 · United States
NIGMS NIH HHS · GM-28310 · United States
Databases
GENBANK
K02436
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