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PMID: 6322136 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The nucleotide sequence of the Escherichia coli fus gene, coding for elongation factor G.

Nucleic acids research ·Vol. 12 ·No. 4 ·1984-02-24 ·Pages 2181-92

Zengel JM, Archer RH, Lindahl L

Abstract

We have determined the nucleotide sequence of the Escherichia coli fus gene, which codes for elongation factor G. The protein product of the sequenced gene contains 703 amino acids, with a predicted molecular weight of 77,444. The fus gene shows the nonrandom pattern of codon usage typical of ribosomal proteins and other proteins synthesized at a high level. We have identified several potential promoter sequences within the gene. One of these sequences may correspond to the secondary promoter for expression of the downstream tufA gene (encoding elongation factor Tu) whose activity has been described previously (1,2). A comparison of the nucleotide and amino acid sequences of elongation factors G and Tu reveals a limited but significant homology between the two proteins within the 150 amino acid residues at their amino-terminal ends.

MeSH Terms
Amino Acid Sequence Base Sequence Codon DNA Restriction Enzymes Escherichia coli/genetics Genes, Bacterial Genes, Fungal Molecular Weight Operon Peptide Elongation Factor G Peptide Elongation Factors/genetics Ribosomal Proteins/genetics
Chemicals
Codon Peptide Elongation Factor G Peptide Elongation Factors Ribosomal Proteins DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Zengel J M
Archer R H
Lindahl L
References (21)
21 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.4.2181
Published
1984-02-24
Pages
2181-92
Language
English
Region
England
NLM ID
0411011
PMCID
PMC318650
Subset
IM
Grants
NIAID NIH HHS · AI 15286 · United States
Databases
GENBANK
X00415
External Links
PubMed source DOI: 10.1093/nar/12.4.2181 PMC Full Text View journal details
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